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Lack of Outer Membrane Protein A Enhances the Release of Outer Membrane Vesicles and Survival of Vibrio cholerae and Suppresses Viability of Acanthamoeba castellanii
Joint Authors
Alossimi, Haifa
Shanan, Salah
Valeru, Soni Priya
Saeed, Amir
Sandström, Gunnar
Abd, Hadi
Source
International Journal of Microbiology
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-01
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Abstract EN
Vibrio cholerae, the causative agent of the diarrhoeal disease cholera, survives in aquatic environments.
The bacterium has developed a survival strategy to grow and survive inside Acanthamoeba castellanii.
It has been shown that V.
cholerae expresses outer membrane proteins as virulence factors playing a role in the adherence to interacted host cells.
This study examined the role of outer membrane protein A (OmpA) and outer membrane vesicles (OMVs) in survival of V.
cholerae alone and during its interaction with A.
castellanii.
The results showed that an OmpA mutant of V.
cholerae survived longer than wild-type V.
cholerae when cultivated alone.
Cocultivation with A.
castellanii enhanced the survival of both bacterial strains and OmpA protein exhibited no effect on attachment, engulfment, and survival inside the amoebae.
However, cocultivation of the OmpA mutant of V.
cholerae decreased the viability of A.
castellanii and this bacterial strain released more OMVs than wild-type V.
cholerae.
Surprisingly, treatment of amoeba cells with OMVs isolated from the OmpA mutant significantly decreased viable counts of the amoeba cells.
In conclusion, the results might highlight a regulating rule for OmpA in survival of V.
cholerae and OMVs as a potent virulence factor for this bacterium towards eukaryotes in the environment.
American Psychological Association (APA)
Valeru, Soni Priya& Shanan, Salah& Alossimi, Haifa& Saeed, Amir& Sandström, Gunnar& Abd, Hadi. 2014. Lack of Outer Membrane Protein A Enhances the Release of Outer Membrane Vesicles and Survival of Vibrio cholerae and Suppresses Viability of Acanthamoeba castellanii. International Journal of Microbiology،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-484912
Modern Language Association (MLA)
Valeru, Soni Priya…[et al.]. Lack of Outer Membrane Protein A Enhances the Release of Outer Membrane Vesicles and Survival of Vibrio cholerae and Suppresses Viability of Acanthamoeba castellanii. International Journal of Microbiology No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-484912
American Medical Association (AMA)
Valeru, Soni Priya& Shanan, Salah& Alossimi, Haifa& Saeed, Amir& Sandström, Gunnar& Abd, Hadi. Lack of Outer Membrane Protein A Enhances the Release of Outer Membrane Vesicles and Survival of Vibrio cholerae and Suppresses Viability of Acanthamoeba castellanii. International Journal of Microbiology. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-484912
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-484912