Nitric Oxide and Superoxide Anion Balance in Rats Exposed to Chronic and Long Term Intermittent Hypoxia
Joint Authors
Arribas, Silvia M.
Flores, Karen
Brito, Julio
González, María Carmen
López, M. Rosario
León-Velarde, Fabiola
Siques, Patricia
Naveas, Nelson
Hoorntje, Alexander
López de Pablo, Ángel Luis
Arriaza, Karem
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-26
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Work at high altitude in shifts exposes humans to a new form of chronic intermittent hypoxia, with still unknown health consequences.
We have established a rat model resembling this situation, which develops a milder form of right ventricular hypertrophy and pulmonary artery remodelling compared to continuous chronic exposure.
We aimed to compare the alterations in pulmonary artery nitric oxide (NO) availability induced by these forms of hypoxia and the mechanisms implicated.
Rats were exposed for 46 days to normoxia or hypobaric hypoxia, either continuous (CH) or intermittent (2 day shifts, CIH2x2), and assessed: NO and superoxide anion availability (fluorescent indicators and confocal microscopy); expression of phosphorylated endothelial NO synthase (eNOS), NADPH-oxidase (p22phox), and 3-nitrotyrosine (western blotting); and NADPH-oxidase location (immunohistochemistry).
Compared to normoxia, (1) NO availability was reduced and superoxide anion was increased in both hypoxic groups, with a larger effect in CH, (2) eNOS expression was only reduced in CH, (3) NADPH-oxidase was similarly increased in both hypoxic groups, and (4) 3-nitrotyrosine was increased to a larger extent in CH.
In conclusion, intermittent hypoxia reduces NO availability through superoxide anion destruction, without reducing its synthesis, while continuous hypoxia affects both, producing larger nitrosative damage which could be related to the more severe cardiovascular alterations.
American Psychological Association (APA)
Siques, Patricia& López de Pablo, Ángel Luis& Brito, Julio& Arribas, Silvia M.& Flores, Karen& Arriaza, Karem…[et al.]. 2014. Nitric Oxide and Superoxide Anion Balance in Rats Exposed to Chronic and Long Term Intermittent Hypoxia. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-484950
Modern Language Association (MLA)
Siques, Patricia…[et al.]. Nitric Oxide and Superoxide Anion Balance in Rats Exposed to Chronic and Long Term Intermittent Hypoxia. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-484950
American Medical Association (AMA)
Siques, Patricia& López de Pablo, Ángel Luis& Brito, Julio& Arribas, Silvia M.& Flores, Karen& Arriaza, Karem…[et al.]. Nitric Oxide and Superoxide Anion Balance in Rats Exposed to Chronic and Long Term Intermittent Hypoxia. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-484950
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-484950