Tanshinone IIA and Cryptotanshinone Prevent Mitochondrial Dysfunction in Hypoxia-Induced H9c2 Cells : Association to Mitochondrial ROS, Intracellular Nitric Oxide, and Calcium Levels
Joint Authors
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-03-04
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The protective actions of tanshinones on hypoxia-induced cell damages have been reported, although the mechanisms have not been fully elucidated.
Given the importance of nitric oxide (NO) and reactive oxygen species (ROS) in regulation of cell functions, the present study investigated the effects of two major tanshinones, Tanshinone IIA (TIIA) and cryptotanshinone (CT), on hypoxia-induced myocardial cell injury and its relationships with intracellular NO and ROS, calcium, and ATP levels in H9c2 cells.
Chronic hypoxia significantly reduced cell viability which accompanied with LDH release, increase in mitochondrial ROS, intracellular NO and calcium levels, decrease in superoxide dismutase (SOD) activity, and cellular ATP contents.
TIIA and CT significantly prevented cell injury by increasing cell viability and decreasing LDH release.
The protective effects of tanshinones were associated with reduced mitochondrial superoxide production and enhanced mitochondrial SOD activity.
Tanshinones significantly reduced intracellular NO and Ca2+ levels.
ATP levels were also restored by TIIA.
These findings suggest that the cytoprotective actions of tanshinones may involve regulation of intracellular NO, Ca2+, ATP productions, mitochondrial superoxide production, and SOD activity, which contribute to their actions against hypoxia injuries.
American Psychological Association (APA)
Jin, Hyou-Ju& Li, Chun Guang. 2013. Tanshinone IIA and Cryptotanshinone Prevent Mitochondrial Dysfunction in Hypoxia-Induced H9c2 Cells : Association to Mitochondrial ROS, Intracellular Nitric Oxide, and Calcium Levels. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-484982
Modern Language Association (MLA)
Jin, Hyou-Ju& Li, Chun Guang. Tanshinone IIA and Cryptotanshinone Prevent Mitochondrial Dysfunction in Hypoxia-Induced H9c2 Cells : Association to Mitochondrial ROS, Intracellular Nitric Oxide, and Calcium Levels. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-484982
American Medical Association (AMA)
Jin, Hyou-Ju& Li, Chun Guang. Tanshinone IIA and Cryptotanshinone Prevent Mitochondrial Dysfunction in Hypoxia-Induced H9c2 Cells : Association to Mitochondrial ROS, Intracellular Nitric Oxide, and Calcium Levels. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-484982
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-484982