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Exploitation of a Very Small Peptide Nucleic Acid as a New Inhibitor of miR-509-3p Involved in the Regulation of Cystic Fibrosis Disease-Gene Expression
Joint Authors
Tomaiuolo, Rossella
De Rosa, Giuseppe
Oliviero, Giorgia
Mayol, Laura
Nici, Fabrizia
Catalanotti, Bruno
Piccialli, Gennaro
Morgillo, Carmine Marco
D'Errico, Stefano
Castaldo, Giuseppe
Borbone, Nicola
Amato, Felice
Elce, Ausilia
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-15
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Computational techniques, and in particular molecular dynamics (MD) simulations, have been successfully used as a complementary technique to predict and analyse the structural behaviour of nucleic acids, including peptide nucleic acid- (PNA-) RNA hybrids.
This study shows that a 7-base long PNA complementary to the seed region of miR-509-3p, one of the miRNAs involved in the posttranscriptional regulation of the CFTR disease-gene of Cystic Fibrosis, and bearing suitable functionalization at its N- and C-ends aimed at improving its resistance to nucleases and cellular uptake, is able to revert the expression of the luciferase gene containing the 3′UTR of the gene in A549 human lung cancer cells, in agreement with the MD results that pointed at the formation of a stable RNA/PNA heteroduplex notwithstanding the short sequence of the latter.
The here reported results widen the interest towards the use of small PNAs as effective anti-miRNA agents.
American Psychological Association (APA)
Amato, Felice& Tomaiuolo, Rossella& Nici, Fabrizia& Borbone, Nicola& Elce, Ausilia& Catalanotti, Bruno…[et al.]. 2014. Exploitation of a Very Small Peptide Nucleic Acid as a New Inhibitor of miR-509-3p Involved in the Regulation of Cystic Fibrosis Disease-Gene Expression. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-484985
Modern Language Association (MLA)
Amato, Felice…[et al.]. Exploitation of a Very Small Peptide Nucleic Acid as a New Inhibitor of miR-509-3p Involved in the Regulation of Cystic Fibrosis Disease-Gene Expression. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-484985
American Medical Association (AMA)
Amato, Felice& Tomaiuolo, Rossella& Nici, Fabrizia& Borbone, Nicola& Elce, Ausilia& Catalanotti, Bruno…[et al.]. Exploitation of a Very Small Peptide Nucleic Acid as a New Inhibitor of miR-509-3p Involved in the Regulation of Cystic Fibrosis Disease-Gene Expression. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-484985
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-484985