Development and Validation of a RP-HPLC Method for Determination of Nimodipine in Sustained Release Tablets

Abstract EN

A rapid, sensitive, and reproducible reverse phase high performance liquid chromatographic (RP-HPLC) method with UV detector for the determination of nimodipine in sustained release tablets was developed.

The method involved using a SinoChoom ODS-BP C18 reversed phase column (5 μm, 4.6 mm × 200 mm) and mobile phase consisting of methanol-acetonitrile-water (35 : 38 : 27, v/v).

The flow rate is 1.0 mL/min, the UV detector was operated at 237 nm, and the column was maintained at 25°C.

The method was validated according to official compendia guidelines.

The calibration curve of nimodipine for RP-HPLC method was linear over the range of 10–100 μg/mL.

The retention time was found at 7.50 min for nimodipine.

The variation for interday and intraday assay was found to be less than 0.72%.

The proposed RP-HPLC was proved to be suitable for the determination of nimodipine in sustained release tablets.