Evaluation of Genotoxic and Cytotoxic Effects in Human Peripheral Blood Lymphocytes Exposed In Vitro to Neonicotinoid Insecticides News
Joint Authors
Martínez-Valenzuela, Carmen
Villalobos-Pietrini, Rafael
Calderón-Segura, María Elena
Gómez-Arroyo, Sandra
García-Martínez, Rocío
Calderón-Ezquerro, María del Carmen
Cortés-Eslava, Josefina
Rodríguez-Romero, María Isabel
Méndez-Pérez, Patricia
Bañuelos-Ruíz, Enrique
Carbajal-López, Yolanda
Flores-Ramírez, Diana
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-03-27
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Pharmacy, Health & Medical Sciences
Medicine
Abstract EN
Calypso (thiacloprid), Poncho (clothianidin), Gaucho (imidacloprid), and Jade (imidacloprid) are commercial neonicotinoid insecticides, a new class of agrochemicals in México.
However, genotoxic and cytotoxic studies have not been performed.
In the present study, human peripheral blood lymphocytes (PBL) were exposed in vitro to different concentrations of the four insecticides.
The genotoxic and cytotoxic effects were evaluated using the alkaline comet and trypan blue dye exclusion assays.
DNA damage was evaluated using two genotoxicity parameters: tail length and comet frequency.
Exposure to 9.5×10-6 to 5.7×10-5 M Jade; 2.8×10-4 to 1.7×10-3 M Gaucho; 0.6×10-1 to 1.4×10-1 M Calypso; 1.2×10-1 to 9.5×10-1 M Poncho for 2 h induced a significant increase DNA damage with a concentration-dependent relationship.
Jade was the most genotoxic of the four insecticides studied.
Cytotoxicity was observed in cells exposed to 18×10-3 M Jade, 2.0×10-3 M Gaucho, 2.0×10-1 M Calypso, 1.07 M Poncho, and cell death occurred at 30×10-3 M Jade, 3.3×10-3 M Gaucho, 2.8×10-1 M Calypso, and 1.42 M Poncho.
This study provides the first report of genotoxic and cytotoxic effects in PBL following in vitro exposure to commercial neonicotinoid insecticides.
American Psychological Association (APA)
Calderón-Segura, María Elena& Gómez-Arroyo, Sandra& Villalobos-Pietrini, Rafael& Martínez-Valenzuela, Carmen& Carbajal-López, Yolanda& Calderón-Ezquerro, María del Carmen…[et al.]. 2012. Evaluation of Genotoxic and Cytotoxic Effects in Human Peripheral Blood Lymphocytes Exposed In Vitro to Neonicotinoid Insecticides News. Journal of Toxicology،Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-485073
Modern Language Association (MLA)
Calderón-Segura, María Elena…[et al.]. Evaluation of Genotoxic and Cytotoxic Effects in Human Peripheral Blood Lymphocytes Exposed In Vitro to Neonicotinoid Insecticides News. Journal of Toxicology No. 2012 (2012), pp.1-11.
https://search.emarefa.net/detail/BIM-485073
American Medical Association (AMA)
Calderón-Segura, María Elena& Gómez-Arroyo, Sandra& Villalobos-Pietrini, Rafael& Martínez-Valenzuela, Carmen& Carbajal-López, Yolanda& Calderón-Ezquerro, María del Carmen…[et al.]. Evaluation of Genotoxic and Cytotoxic Effects in Human Peripheral Blood Lymphocytes Exposed In Vitro to Neonicotinoid Insecticides News. Journal of Toxicology. 2012. Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-485073
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-485073