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A Regulatory Role of NAD Redox Status on Flavin Cofactor Homeostasis in S. cerevisiae Mitochondria
Joint Authors
Locato, Vittoria
Giancaspero, Teresa Anna
Barile, Maria
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-01
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Flavin adenine dinucleotide (FAD) and nicotinamide adenine dinucleotide (NAD) are two redox cofactors of pivotal importance for mitochondrial functionality and cellular redox balance.
Despite their relevance, the mechanism by which intramitochondrial NAD(H) and FAD levels are maintained remains quite unclear in Saccharomyces cerevisiae.
We investigated here the ability of isolated mitochondria to degrade externally added FAD and NAD (in both its reduced and oxidized forms).
A set of kinetic experiments demonstrated that mitochondrial FAD and NAD(H) destroying enzymes are different from each other and from the already characterized NUDIX hydrolases.
We studied here, in some detail, FAD pyrophosphatase (EC 3.6.1.18), which is inhibited by NAD+ and NADH according to a noncompetitive inhibition, with Ki values that differ from each other by an order of magnitude.
These findings, together with the ability of mitochondrial FAD pyrophosphatase to metabolize endogenous FAD, presumably deriving from mitochondrial holoflavoproteins destined to degradation, allow for proposing a novel possible role of mitochondrial NAD redox status in regulating FAD homeostasis and/or flavoprotein degradation in S.
cerevisiae.
American Psychological Association (APA)
Giancaspero, Teresa Anna& Locato, Vittoria& Barile, Maria. 2013. A Regulatory Role of NAD Redox Status on Flavin Cofactor Homeostasis in S. cerevisiae Mitochondria. Oxidative Medicine and Cellular Longevity،Vol. 2013, no. 2013, pp.1-16.
https://search.emarefa.net/detail/BIM-485090
Modern Language Association (MLA)
Giancaspero, Teresa Anna…[et al.]. A Regulatory Role of NAD Redox Status on Flavin Cofactor Homeostasis in S. cerevisiae Mitochondria. Oxidative Medicine and Cellular Longevity No. 2013 (2013), pp.1-16.
https://search.emarefa.net/detail/BIM-485090
American Medical Association (AMA)
Giancaspero, Teresa Anna& Locato, Vittoria& Barile, Maria. A Regulatory Role of NAD Redox Status on Flavin Cofactor Homeostasis in S. cerevisiae Mitochondria. Oxidative Medicine and Cellular Longevity. 2013. Vol. 2013, no. 2013, pp.1-16.
https://search.emarefa.net/detail/BIM-485090
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-485090