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Disruption of HPV16-E7 by CRISPRCas System Induces Apoptosis and Growth Inhibition in HPV16 Positive Human Cervical Cancer Cells
Joint Authors
Hu, Zheng
Wang, Hui
Shen, Hui
He, Dan
Ma, Ding
Li, Kezhen
Ding, Wencheng
Wang, Liming
Zhang, Changlin
Zhu, Da
Jiang, Xiaohui
Wang, Xiaoli
Yu, Lan
Xi, Ling
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-20
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
High-risk human papillomavirus (HR-HPV) has been recognized as a major causative agent for cervical cancer.
Upon HPV infection, early genes E6 and E7 play important roles in maintaining malignant phenotype of cervical cancer cells.
By using clustered regularly interspaced short palindromic repeats- (CRISPR-) associated protein system (CRISPR/Cas system), a widely used genome editing tool in many organisms, to target HPV16-E7 DNA in HPV positive cell lines, we showed for the first time that the HPV16-E7 single-guide RNA (sgRNA) guided CRISPR/Cas system could disrupt HPV16-E7 DNA at specific sites, inducing apoptosis and growth inhibition in HPV positive SiHa and Caski cells, but not in HPV negative C33A and HEK293 cells.
Moreover, disruption of E7 DNA directly leads to downregulation of E7 protein and upregulation of tumor suppressor protein pRb.
Therefore, our results suggest that HPV16-E7 gRNA guided CRISPR/Cas system might be used as a therapeutic strategy for the treatment of cervical cancer.
American Psychological Association (APA)
Hu, Zheng& Yu, Lan& Zhu, Da& Ding, Wencheng& Wang, Xiaoli& Zhang, Changlin…[et al.]. 2014. Disruption of HPV16-E7 by CRISPRCas System Induces Apoptosis and Growth Inhibition in HPV16 Positive Human Cervical Cancer Cells. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-485096
Modern Language Association (MLA)
Hu, Zheng…[et al.]. Disruption of HPV16-E7 by CRISPRCas System Induces Apoptosis and Growth Inhibition in HPV16 Positive Human Cervical Cancer Cells. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-485096
American Medical Association (AMA)
Hu, Zheng& Yu, Lan& Zhu, Da& Ding, Wencheng& Wang, Xiaoli& Zhang, Changlin…[et al.]. Disruption of HPV16-E7 by CRISPRCas System Induces Apoptosis and Growth Inhibition in HPV16 Positive Human Cervical Cancer Cells. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-485096
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-485096