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A Cotton-Fiber-Associated Cyclin-Dependent Kinase A Gene : Characterization and Chromosomal Location
Joint Authors
Ma, Din-Pow
Jenkins, Johnie N.
Saha, Sukumar
Gao, Weifan
Stelly, David M.
Guo, Yufang
Source
International Journal of Plant Genomics
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-06-14
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
A cotton fiber cDNA and its genomic sequences encoding an A-type cyclin-dependent kinase (GhCDKA) were cloned and characterized.
The encoded GhCDKA protein contains the conserved cyclin-binding, ATP binding, and catalytic domains.
Northern blot and RT-PCR analysis revealed that the GhCDKA transcript was high in 5–10 DPA fibers, moderate in 15 and 20 DPA fibers and roots, and low in flowers and leaves.
GhCDKA protein levels in fibers increased from 5–15 DPA, peaked at 15 DPA, and decreased from 15 t0 20 DPA.
The differential expression of GhCDKA suggested that the gene might play an important role in fiber development.
The GhCDKA sequence data was used to develop single nucleotide polymorphism (SNP) markers specific for the CDKA gene in cotton.
A primer specific to one of the SNPs was used to locate the CDKA gene to chromosome 16 by deletion analysis using a series of hypoaneuploid interspecific hybrids.
American Psychological Association (APA)
Gao, Weifan& Saha, Sukumar& Ma, Din-Pow& Guo, Yufang& Jenkins, Johnie N.& Stelly, David M.. 2012. A Cotton-Fiber-Associated Cyclin-Dependent Kinase A Gene : Characterization and Chromosomal Location. International Journal of Plant Genomics،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-485171
Modern Language Association (MLA)
Gao, Weifan…[et al.]. A Cotton-Fiber-Associated Cyclin-Dependent Kinase A Gene : Characterization and Chromosomal Location. International Journal of Plant Genomics No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-485171
American Medical Association (AMA)
Gao, Weifan& Saha, Sukumar& Ma, Din-Pow& Guo, Yufang& Jenkins, Johnie N.& Stelly, David M.. A Cotton-Fiber-Associated Cyclin-Dependent Kinase A Gene : Characterization and Chromosomal Location. International Journal of Plant Genomics. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-485171
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-485171