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Mechanisms of Ascorbyl Radical Formation in Human Platelet-Rich Plasma
Joint Authors
Yeh, Yu-Chieh
Shyu, Kou-Gi
Chou, Duen-Suey
Sheu, Joen-Rong
Chang, Chao-Chien
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-17
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Recently, many clinical reports have suggested that the ascorbyl free radical (Asc∙) can be treated as a noninvasive, reliable, real-time marker of oxidative stress, but its generation mechanisms in human blood have rarely been discussed.
In this study, we used upstream substances, enzyme inhibitors, and free radical scavengers to delineate the mechanisms of Asc∙ formation in human platelet-rich plasma (PRP).
Our results show that the doublet signal was detected in PRP samples by using electron spin resonance, and the hyperfine splitting of the doublet signal was aH=1.88 gauss and g-factor = 2.00627, which was determined to be the Asc∙.
We observed that the inhibitors of NADPH oxidase (NOX), cyclooxygenase (COX), lipoxygenase (LOX), cytochrome P450 (CYP450), mitochondria complex III, and nitric oxide synthase (NOS), but not xanthine oxidase, diminished the intensity of the Asc∙ signal dose dependently.
All enzyme inhibitors showed no obvious antioxidant activity during a Fenton reaction assay.
In summary, the obtained data suggest that Asc∙ formation is associated with NOX, COX, LOX, CYP450, eNOS, and mitochondria in human PRP.
American Psychological Association (APA)
Shyu, Kou-Gi& Chang, Chao-Chien& Yeh, Yu-Chieh& Sheu, Joen-Rong& Chou, Duen-Suey. 2014. Mechanisms of Ascorbyl Radical Formation in Human Platelet-Rich Plasma. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-485241
Modern Language Association (MLA)
Shyu, Kou-Gi…[et al.]. Mechanisms of Ascorbyl Radical Formation in Human Platelet-Rich Plasma. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-485241
American Medical Association (AMA)
Shyu, Kou-Gi& Chang, Chao-Chien& Yeh, Yu-Chieh& Sheu, Joen-Rong& Chou, Duen-Suey. Mechanisms of Ascorbyl Radical Formation in Human Platelet-Rich Plasma. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-485241
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-485241