High Level Expression and Purification of Atl, the Major Autolytic Protein of Staphylococcus aureus
Author
Source
International Journal of Microbiology
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-19
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Abstract EN
Staphylococcus aureus is a major human and animal pathogen.
Autolysins regulate the growth, turnover, cell lysis, biofilm formation, and the pathogenicity of S.
aureus.
Atl is the major autolysin in S.
aureus.
The biochemical and structural studies of staphylococcal Atl have been limited due to difficulty in cloning, high level overexpression, and purification of this protein.
This study describes successful cloning, high level over-expression, and purification of two forms of fully functional Atl proteins.
These pure proteins can be used to study the functional and structural properties of this important protein.
American Psychological Association (APA)
Singh, Vineet K.. 2014. High Level Expression and Purification of Atl, the Major Autolytic Protein of Staphylococcus aureus. International Journal of Microbiology،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-485369
Modern Language Association (MLA)
Singh, Vineet K.. High Level Expression and Purification of Atl, the Major Autolytic Protein of Staphylococcus aureus. International Journal of Microbiology No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-485369
American Medical Association (AMA)
Singh, Vineet K.. High Level Expression and Purification of Atl, the Major Autolytic Protein of Staphylococcus aureus. International Journal of Microbiology. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-485369
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-485369