Immunogenicity and Protective Efficacy against Murine Tuberculosis of a Prime-Boost Regimen with BCG and a DNA Vaccine Expressing ESAT-6 and Ag85A Fusion Protein
Joint Authors
Zhang, Ying
Chen, Zhenhua
Fan, Xionglin
Wang, Chun
Zhou, Zhiguang
Lu, Jia
Cao, Tingting
Chen, Lingxia
Shi, Chunwei
Cai, Changxue
Source
Clinical and Developmental Immunology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-02-27
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Heterologous prime-boost regimens utilizing BCG as a prime vaccine probably represent the best hope for the development of novel tuberculosis (TB) vaccines.
In this study, we examined the immunogenicity and protective efficacy of DNA vaccine (pcD685A) expressing the fusion protein of Ag85A and ESAT-6 (r685A) and its booster effects in BCG-immunized mice.
The recombinant r685A fusion protein stimulated higher level of antigen-specific IFN-γ release in tuberculin skin test- (TST-) positive healthy household contacts of active pulmonary TB patients than that in TST-negative population.
Vaccination of C57BL/6 mice with pcD685A resulted in significant protection against challenge with virulent Mycobacterium tuberculosis H37Rv when compared with the control group.
Most importantly, pcD685A could act as a BCG booster and amplify Th1-type cell-mediated immunity in the lung of BCG-vaccinated mice as shown the increased expression of IFN-γ.
The most significant reduction in bacterial load of both spleen and lung was obtained in mice vaccinated with BCG prime and pcD685A DNA booster when compared with BCG or pcD685A alone.
Thus, our study indicates that pcD685A may be an efficient booster vaccine against TB with a strong ability to enhance prior BCG immunity.
American Psychological Association (APA)
Lu, Jia& Wang, Chun& Zhou, Zhiguang& Zhang, Ying& Cao, Tingting& Shi, Chunwei…[et al.]. 2011. Immunogenicity and Protective Efficacy against Murine Tuberculosis of a Prime-Boost Regimen with BCG and a DNA Vaccine Expressing ESAT-6 and Ag85A Fusion Protein. Clinical and Developmental Immunology،Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-485533
Modern Language Association (MLA)
Lu, Jia…[et al.]. Immunogenicity and Protective Efficacy against Murine Tuberculosis of a Prime-Boost Regimen with BCG and a DNA Vaccine Expressing ESAT-6 and Ag85A Fusion Protein. Clinical and Developmental Immunology No. 2011 (2011), pp.1-10.
https://search.emarefa.net/detail/BIM-485533
American Medical Association (AMA)
Lu, Jia& Wang, Chun& Zhou, Zhiguang& Zhang, Ying& Cao, Tingting& Shi, Chunwei…[et al.]. Immunogenicity and Protective Efficacy against Murine Tuberculosis of a Prime-Boost Regimen with BCG and a DNA Vaccine Expressing ESAT-6 and Ag85A Fusion Protein. Clinical and Developmental Immunology. 2011. Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-485533
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-485533