Photoactivatable Fluorophores
Author
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-09-17
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Photoactivatable fluorophores switch from a nonemissive to an emissive state upon illumination at an activating wavelength and then emit after irradiation at an exciting wavelength.
The interplay of such activation and excitation events can be exploited to switch fluorescence on in a defined region of space at a given interval of time.
In turn, the spatiotemporal control of fluorescence translates into the opportunity to implement imaging and spectroscopic schemes that are not possible with conventional fluorophores.
Specifically, photoactivatable fluorophores permit the monitoring of dynamic processes in real time as well as the reconstruction of images with subdiffraction resolution.
These promising applications can have a significant impact on the characterization of the structures and functions of biomolecular systems.
As a result, strategies to implement mechanisms for fluorescence photoactivation with synthetic fluorophores are particularly valuable.
In fact, a number of versatile operating principles have already been identified to activate the fluorescence of numerous members of the main families of synthetic dyes.
These methods are based on either the irreversible cleavage of covalent bonds or the reversible opening and closing of rings.
This paper overviews the fundamental mechanisms that govern the behavior of these photoresponsive systems, illustrates structural designs for fluorescence photoactivation, and provides representative examples of photoactivatable fluorophores in actions.
American Psychological Association (APA)
Raymo, Françisco M.. 2012. Photoactivatable Fluorophores. ISRN Physical Chemistry،Vol. 2012, no. 2012, pp.1-15.
https://search.emarefa.net/detail/BIM-485662
Modern Language Association (MLA)
Raymo, Françisco M.. Photoactivatable Fluorophores. ISRN Physical Chemistry No. 2012 (2012), pp.1-15.
https://search.emarefa.net/detail/BIM-485662
American Medical Association (AMA)
Raymo, Françisco M.. Photoactivatable Fluorophores. ISRN Physical Chemistry. 2012. Vol. 2012, no. 2012, pp.1-15.
https://search.emarefa.net/detail/BIM-485662
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-485662