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Electrochemical Detection of Sequence-Specific DNA with the Amplification of Gold Nanoparticles
Joint Authors
Jiang, Wei
Huang, Lei
Wang, Mingzhu
Wang, Zhen
Wang, Yuehong
Zhang, Yuzhong
Source
International Journal of Electrochemistry
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-05-29
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
A sensitive electrochemical DNA biosensor was prepared based on mercaptoacetic acid (MAA)/gold nanoparticles (AuNPs) modified electrode.
Probe DNA (NH2-DNA) was covalently linked to the carboxyl group of MAA in the presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxyl-succinimide (NHS).
Scanning electron microscopy (SEM) and electrochemical impedance spectra (EIS) were used to investigate the film assembly process.
The DNA hybridization events were monitored by differential pulse voltammetry (DPV), and adriamycin was used as the electrochemical indicator.
Also the factors influencing the performance of the DNA hybridization were investigated in detail.
Under the optimal conditions, the signal was linearly changed with target DNA concentration increased from 5.0 × 10−13 to 1.0 × 10−9 M and had a detection limit of 1.7 × 10−13 M (signal/noise ratio of 3).
In addition, the DNA biosensor showed good reproducibility and stability during DNA assay.
American Psychological Association (APA)
Zhang, Yuzhong& Wang, Zhen& Wang, Yuehong& Huang, Lei& Jiang, Wei& Wang, Mingzhu. 2011. Electrochemical Detection of Sequence-Specific DNA with the Amplification of Gold Nanoparticles. International Journal of Electrochemistry،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-485716
Modern Language Association (MLA)
Zhang, Yuzhong…[et al.]. Electrochemical Detection of Sequence-Specific DNA with the Amplification of Gold Nanoparticles. International Journal of Electrochemistry No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-485716
American Medical Association (AMA)
Zhang, Yuzhong& Wang, Zhen& Wang, Yuehong& Huang, Lei& Jiang, Wei& Wang, Mingzhu. Electrochemical Detection of Sequence-Specific DNA with the Amplification of Gold Nanoparticles. International Journal of Electrochemistry. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-485716
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-485716