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Effect of Decellularization Protocol on the Mechanical Behavior of Porcine Descending Aorta
Joint Authors
Fitzpatrick, John C.
Capaldi, Franco M.
Clark, Peter M.
Source
International Journal of Biomaterials
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-07-04
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Enzymatic-detergent decellularization treatments may use a combination of chemical reagents to reduce vascular tissue to sterilized scaffolds, which may be seeded with endothelial cells and implanted with a low risk of rejection.
However, these chemicals may alter the mechanical properties of the native tissue and contribute to graft compliance mismatch.
Uniaxial tensile data obtained from native and decellularized longitudinal aortic tissue samples was analyzed in terms of engineering stress and fit to a modified form of the Yeoh rubber model.
One decellularization protocol used SDS, while the other two used TritonX-100, RNase-A, and DNase-I in combination with EDTA or sodium-deoxycholate.
Statistical significance of Yeoh model parameters was determined by paired t-test analysis.
The TritonX-100/EDTA and 0.075% SDS treatments resulted in relatively variable mechanical changes and did not effectively lyse VSMCs in aortic tissue.
The TritonX-100/sodium-deoxycholate treatment effectively lysed VSMCs and was characterized by less variability in mechanical behavior.
The data suggests a TritonX-100/sodium-deoxycholate treatment is a more effective option than TritonX-100/EDTA and SDS treatments for the preparation of aortic xenografts and allografts because it effectively lyses VSMCs and is the least likely treatment, among those considered, to promote a decrease in mechanical compliance.
American Psychological Association (APA)
Fitzpatrick, John C.& Clark, Peter M.& Capaldi, Franco M.. 2010. Effect of Decellularization Protocol on the Mechanical Behavior of Porcine Descending Aorta. International Journal of Biomaterials،Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-485766
Modern Language Association (MLA)
Fitzpatrick, John C.…[et al.]. Effect of Decellularization Protocol on the Mechanical Behavior of Porcine Descending Aorta. International Journal of Biomaterials No. 2010 (2010), pp.1-11.
https://search.emarefa.net/detail/BIM-485766
American Medical Association (AMA)
Fitzpatrick, John C.& Clark, Peter M.& Capaldi, Franco M.. Effect of Decellularization Protocol on the Mechanical Behavior of Porcine Descending Aorta. International Journal of Biomaterials. 2010. Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-485766
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-485766