Structural Biology of DNA Repair : Spatial Organisation of the Multicomponent Complexes of Nonhomologous End Joining
Joint Authors
Ochi, Takashi
Sibanda, Bancinyane Lynn
Chirgadze, Dimitri Y.
Blundell, Tom L.
Bolanos-Garcia, Victor M.
Wu, Qian
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-19, 19 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-08-25
Country of Publication
Egypt
No. of Pages
19
Main Subjects
Abstract EN
Nonhomologous end joining (NHEJ) plays a major role in double-strand break DNA repair, which involves a series of steps mediated by multiprotein complexes.
A ring-shaped Ku70/Ku80 heterodimer forms first at broken DNA ends, DNA-dependent protein kinase catalytic subunit (DNA-PKcs) binds to mediate synapsis and nucleases process DNA overhangs.
DNA ligase IV (LigIV) is recruited as a complex with XRCC4 for ligation, with XLF/Cernunnos, playing a role in enhancing activity of LigIV.
We describe how a combination of methods—X-ray crystallography, electron microscopy and small angle X-ray scattering—can give insights into the transient multicomponent complexes that mediate NHEJ.
We first consider the organisation of DNA-PKcs/Ku70/Ku80/DNA complex (DNA-PK) and then discuss emerging evidence concerning LigIV/XRCC4/XLF/DNA and higher-order complexes.
We conclude by discussing roles of multiprotein systems in maintaining high signal-to-noise and the value of structural studies in developing new therapies in oncology and elsewhere.
American Psychological Association (APA)
Ochi, Takashi& Sibanda, Bancinyane Lynn& Wu, Qian& Chirgadze, Dimitri Y.& Bolanos-Garcia, Victor M.& Blundell, Tom L.. 2010. Structural Biology of DNA Repair : Spatial Organisation of the Multicomponent Complexes of Nonhomologous End Joining. Journal of Nucleic Acids،Vol. 2010, no. 2010, pp.1-19.
https://search.emarefa.net/detail/BIM-485887
Modern Language Association (MLA)
Ochi, Takashi…[et al.]. Structural Biology of DNA Repair : Spatial Organisation of the Multicomponent Complexes of Nonhomologous End Joining. Journal of Nucleic Acids No. 2010 (2010), pp.1-19.
https://search.emarefa.net/detail/BIM-485887
American Medical Association (AMA)
Ochi, Takashi& Sibanda, Bancinyane Lynn& Wu, Qian& Chirgadze, Dimitri Y.& Bolanos-Garcia, Victor M.& Blundell, Tom L.. Structural Biology of DNA Repair : Spatial Organisation of the Multicomponent Complexes of Nonhomologous End Joining. Journal of Nucleic Acids. 2010. Vol. 2010, no. 2010, pp.1-19.
https://search.emarefa.net/detail/BIM-485887
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-485887