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Optimization of Fermentation Medium for the Production of Atrazine Degrading Strain Acinetobacter sp. DNS32 by Statistical Analysis System
Joint Authors
Meng, Dong-Fang
Zhang, Ying
Wang, Xi
Guo, Huo-Sheng
Wang, Yang
Wong, Po-keung
Wang, Zhi-Gang
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-10-03
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Statistical experimental designs provided by statistical analysis system (SAS) software were applied to optimize the fermentation medium composition for the production of atrazine-degrading Acinetobacter sp.
DNS32 in shake-flask cultures.
A “Plackett-Burman Design” was employed to evaluate the effects of different components in the medium.
The concentrations of corn flour, soybean flour, and K2HPO4 were found to significantly influence Acinetobacter sp.
DNS32 production.
The steepest ascent method was employed to determine the optimal regions of these three significant factors.
Then, these three factors were optimized using central composite design of “response surface methodology.” The optimized fermentation medium composition was composed as follows (g/L): corn flour 39.49, soybean flour 25.64, CaCO3 3, K2HPO4 3.27, MgSO4 ·7H2O 0.2, and NaCl 0.2.
The predicted and verifiable values in the medium with optimized concentration of components in shake flasks experiments were 7.079×108 CFU/mL and 7.194×108 CFU/mL, respectively.
The validated model can precisely predict the growth of atrazine-degraing bacterium, Acinetobacter sp.
DNS32.
American Psychological Association (APA)
Zhang, Ying& Wang, Yang& Wang, Zhi-Gang& Wang, Xi& Guo, Huo-Sheng& Meng, Dong-Fang…[et al.]. 2012. Optimization of Fermentation Medium for the Production of Atrazine Degrading Strain Acinetobacter sp. DNS32 by Statistical Analysis System. Journal of Biomedicine and Biotechnology،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-485923
Modern Language Association (MLA)
Zhang, Ying…[et al.]. Optimization of Fermentation Medium for the Production of Atrazine Degrading Strain Acinetobacter sp. DNS32 by Statistical Analysis System. Journal of Biomedicine and Biotechnology No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-485923
American Medical Association (AMA)
Zhang, Ying& Wang, Yang& Wang, Zhi-Gang& Wang, Xi& Guo, Huo-Sheng& Meng, Dong-Fang…[et al.]. Optimization of Fermentation Medium for the Production of Atrazine Degrading Strain Acinetobacter sp. DNS32 by Statistical Analysis System. Journal of Biomedicine and Biotechnology. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-485923
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-485923