The Application of a Three-Step Serum Proteome Analysis for the Discovery and Identification of Novel Biomarkers of Hepatocellular Carcinoma
Joint Authors
Kodera, Yoshio
Yokosuka, Osamu
Kimura, Asako
Sogawa, Kazuyuki
Satoh, Mamoru
Nomura, Fumio
Tomonaga, Takeshi
Source
International Journal of Proteomics
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-08-16
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
The representative tumor markers for HCC, AFP, and PIVKA-II are not satisfactory in terms of sensitivity and specificity in the early diagnosis of HCC.
In search for novel markers for HCC, three-step proteome analyses were carried out in serum samples obtained from 12 patients with HCC and 10 with LC.
As a first step, serum samples were subjected to antibody-based immunoaffinity column system that simultaneously removes twelve of abundant serum proteins.
The concentrated flow-through was then fractionated using reversed-phase HPLC.
Proteins obtained in each fraction were separated by SDS-PAGE.
Serum samples obtained from patient with HCC and with LC were analyzed in parallel and their protein expression patterns were compared.
A total of 83 protein bands were found to be upregulated in HCC serum.
All the protein bands, the intensity of which was different between HCC and LC groups, were identified.
Among them, clusterin was most significantly overexpressed (P=0.023).
The overexpression of serum clusterin was confirmed by ELISA using another validation set of HCC samples.
Furthermore, serum clusterin was elevated in 40% of HCC cases in which both AFP and PIVKA-II were within their cut-off values.
These results suggested that clusterin is a potential novel serum marker for HCC.
American Psychological Association (APA)
Kimura, Asako& Sogawa, Kazuyuki& Satoh, Mamoru& Kodera, Yoshio& Yokosuka, Osamu& Tomonaga, Takeshi…[et al.]. 2012. The Application of a Three-Step Serum Proteome Analysis for the Discovery and Identification of Novel Biomarkers of Hepatocellular Carcinoma. International Journal of Proteomics،Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-485943
Modern Language Association (MLA)
Kimura, Asako…[et al.]. The Application of a Three-Step Serum Proteome Analysis for the Discovery and Identification of Novel Biomarkers of Hepatocellular Carcinoma. International Journal of Proteomics No. 2012 (2012), pp.1-12.
https://search.emarefa.net/detail/BIM-485943
American Medical Association (AMA)
Kimura, Asako& Sogawa, Kazuyuki& Satoh, Mamoru& Kodera, Yoshio& Yokosuka, Osamu& Tomonaga, Takeshi…[et al.]. The Application of a Three-Step Serum Proteome Analysis for the Discovery and Identification of Novel Biomarkers of Hepatocellular Carcinoma. International Journal of Proteomics. 2012. Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-485943
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-485943