Identification of Putative Ortholog Gene Blocks Involved in Gestant and Lactating Mammary Gland Development : A Rodent Cross-Species Microarray Transcriptomics Approach

Joint Authors

Hernández-Stengele, Gabriel
Rodríguez-Cruz, Maricela
Sanchez-Muñoz, Fausto
Encarnación-Guevara, Sergio
Ramírez-Salcedo, Jorge
Salazar, Emmanuel
Sánchez, Raúl
Coral-Vázquez, Ramón M.

Source

International Journal of Genomics

Issue

Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-12, 12 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2013-10-30

Country of Publication

Egypt

No. of Pages

12

Main Subjects

Natural & Life Sciences (Multidisciplinary)
Biology

Abstract EN

The mammary gland (MG) undergoes functional and metabolic changes during the transition from pregnancy to lactation, possibly by regulation of conserved genes.

The objective was to elucidate orthologous genes, chromosome clusters and putative conserved transcriptional modules during MG development.

We analyzed expression of 22,000 transcripts using murine microarrays and RNA samples of MG from virgin, pregnant, and lactating rats by cross-species hybridization.

We identified 521 transcripts differentially expressed; upregulated in early (78%) and midpregnancy (89%) and early lactation (64%), but downregulated in mid-lactation (61%).

Putative orthologous genes were identified.

We mapped the altered genes to orthologous chromosomal locations in human and mouse.

Eighteen sets of conserved genes associated with key cellular functions were revealed and conserved transcription factor binding site search entailed possible coregulation among all eight block sets of genes.

This study demonstrates that the use of heterologous array hybridization for screening of orthologous gene expression from rat revealed sets of conserved genes arranged in chromosomal order implicated in signaling pathways and functional ontology.

Results demonstrate the utilization power of comparative genomics and prove the feasibility of using rodent microarrays to identification of putative coexpressed orthologous genes involved in the control of human mammary gland development.

American Psychological Association (APA)

Rodríguez-Cruz, Maricela& Coral-Vázquez, Ramón M.& Hernández-Stengele, Gabriel& Sánchez, Raúl& Salazar, Emmanuel& Sanchez-Muñoz, Fausto…[et al.]. 2013. Identification of Putative Ortholog Gene Blocks Involved in Gestant and Lactating Mammary Gland Development : A Rodent Cross-Species Microarray Transcriptomics Approach. International Journal of Genomics،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-486060

Modern Language Association (MLA)

Rodríguez-Cruz, Maricela…[et al.]. Identification of Putative Ortholog Gene Blocks Involved in Gestant and Lactating Mammary Gland Development : A Rodent Cross-Species Microarray Transcriptomics Approach. International Journal of Genomics No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-486060

American Medical Association (AMA)

Rodríguez-Cruz, Maricela& Coral-Vázquez, Ramón M.& Hernández-Stengele, Gabriel& Sánchez, Raúl& Salazar, Emmanuel& Sanchez-Muñoz, Fausto…[et al.]. Identification of Putative Ortholog Gene Blocks Involved in Gestant and Lactating Mammary Gland Development : A Rodent Cross-Species Microarray Transcriptomics Approach. International Journal of Genomics. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-486060

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-486060