Immunohistochemical Characterization of Three Monoclonal Antibodies Raised against the Epidermal Growth Factor and Its Receptor in Non-Small-Cell Lung Cancer : Their Potential Use in the Selection of Patients for Immunotherapy
Joint Authors
Calzado, Enrique Rengifo
Cedeño, Mercedes
Rengifo, Charles E.
Blanco, Rancés
Frómeta, Milagros
Blanco, Damián
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-11
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Adequate methods to identify which lung cancer patients are most likely to benefit from the targeted drugs against both epidermal growth factor receptor/epidermal growth factor (EGFR/EGF) are needed.
For this reason, we evaluated both the tissue reactivity of ior egf/r3 monoclonal antibody (Mab) in human lung carcinomas and its biological activity in NCI-H125 cells.
Additionally, we assessed the tissue expression of EGF using two Mabs, CB-EGF1 and CB-EGF2.
The overexpression of EGFR was detected in 33.33% and 62.71% of small-cell lung carcinoma (SCLC) and non-small-cell lung carcinoma (NSCLC), respectively.
The ability of ior egf/r3 Mab to bind the extracellular domain of EGFR inhibiting cell proliferation and inducing apoptosis in NCI-H125 cells was also demonstrated.
The EGF expression was observed in about 17% and 70% of SCLC and NSCLC, respectively.
However, differences in the reactivity of CB-EGF1 and CB-EGF2 were evidenced.
A dual expression of EGFR and EGF was observed in 16.67% and 57.63% of SCLC and NSCLC patients, respectively.
But, a correlation between them was only obtained in NSCLC.
Our results permit to recommend the development of diagnostic kits using ior egf/r3 and/or CB-EGF1 Mabs in order to achieve a better selection of patients to EGFR/EGF-targeting treatment.
American Psychological Association (APA)
Rengifo, Charles E.& Blanco, Rancés& Blanco, Damián& Cedeño, Mercedes& Frómeta, Milagros& Calzado, Enrique Rengifo. 2012. Immunohistochemical Characterization of Three Monoclonal Antibodies Raised against the Epidermal Growth Factor and Its Receptor in Non-Small-Cell Lung Cancer : Their Potential Use in the Selection of Patients for Immunotherapy. Journal of Biomarkers،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-486318
Modern Language Association (MLA)
Rengifo, Charles E.…[et al.]. Immunohistochemical Characterization of Three Monoclonal Antibodies Raised against the Epidermal Growth Factor and Its Receptor in Non-Small-Cell Lung Cancer : Their Potential Use in the Selection of Patients for Immunotherapy. Journal of Biomarkers No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-486318
American Medical Association (AMA)
Rengifo, Charles E.& Blanco, Rancés& Blanco, Damián& Cedeño, Mercedes& Frómeta, Milagros& Calzado, Enrique Rengifo. Immunohistochemical Characterization of Three Monoclonal Antibodies Raised against the Epidermal Growth Factor and Its Receptor in Non-Small-Cell Lung Cancer : Their Potential Use in the Selection of Patients for Immunotherapy. Journal of Biomarkers. 2012. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-486318
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-486318