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Trypanocidal Activity of Smallanthus sonchifolius : Identification of Active Sesquiterpene Lactones by Bioassay-Guided Fractionation
Joint Authors
Malchiodi, Emilio
Ulloa, J.
Maravilla, G.
Martino, Virginia S.
Frank, Fernanda M.
Muschietti, Liliana V.
Cazorla, S. I.
Catalán, César A. N.
Grau, A.
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-06-06
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
In order to find novel plant-derived biologically active compounds against Trypanosoma cruzi, we isolated, from the organic extract of Smallanthus sonchifolius, the sesquiterpene lactones enhydrin (1), uvedalin (2), and polymatin B (3) by bioassay-guided fractionation technique.
These compounds showed a significant trypanocidal activity against the epimastigote forms of the parasite with IC50 values of 0.84 μM (1), 1.09 μM (2), and 4.90 μM (3).
After a 24 h treatment with 10 μg/mL of enhydrin or uvedalin, parasites were not able to recover their replication rate.
Compounds 1 and 2 showed IC50 values of 33.4 μM and 25.0 μM against T.
cruzi trypomastigotes, while polymatin B was not active.
When the three compounds were tested against the intracellular forms of T.
cruzi, they were able to inhibit the amastigote replication with IC50 of 5.17 μM, 3.34 μM, and 9.02 μM for 1, 2, and 3, respectively.
The cytotoxicity of the compounds was evaluated in Vero cells obtaining CC50 values of 46.5 μM (1), 46.8 μM (2), and 147.3 μM (3) and the selectivity index calculated.
According to these results, enhydrin and uvedalin might have potentials as agents against Chagas disease and could serve as lead molecules to develop new drugs.
American Psychological Association (APA)
Frank, Fernanda M.& Ulloa, J.& Cazorla, S. I.& Maravilla, G.& Malchiodi, Emilio& Grau, A.…[et al.]. 2013. Trypanocidal Activity of Smallanthus sonchifolius : Identification of Active Sesquiterpene Lactones by Bioassay-Guided Fractionation. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-486325
Modern Language Association (MLA)
Frank, Fernanda M.…[et al.]. Trypanocidal Activity of Smallanthus sonchifolius : Identification of Active Sesquiterpene Lactones by Bioassay-Guided Fractionation. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-486325
American Medical Association (AMA)
Frank, Fernanda M.& Ulloa, J.& Cazorla, S. I.& Maravilla, G.& Malchiodi, Emilio& Grau, A.…[et al.]. Trypanocidal Activity of Smallanthus sonchifolius : Identification of Active Sesquiterpene Lactones by Bioassay-Guided Fractionation. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-486325
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-486325