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Expression of a Gene Encoding 34.9 kDa PPE Antigen of Mycobacterium avium subsp. paratuberculosis in E. coli
Joint Authors
Source
Molecular Biology International
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-05-18
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Mycobacterium avium subsp.
paratuberculosis (Map) contains PPE family antigens which are Proline and glutamic acid rich and may play important role as T cell antigens.
Hence the identification and generation of antigens are necessary for immunological characterization.
In the present study, the epitopic region of a unique PPE gene encoding 34.9 kDa protein from Map was amplified by polymerase chain reaction.
The gene was cloned into Escherichia coli vector pQE30 UA.
The recombinant plasmid designated as pQPPE was transformed into E.
coli M15 and induced with IPTG revealed the high level expression of 37.1 kDa His-fusion protein (34.9 kDa PPE and 2.2 kDa His-tag), which was confirmed by immunoblotting.
Recombinant PPE protein was then purified by Ni-NTA agarose chromatography.
The polyclonal antiserum raised against purified recombinant PPE protein reacted with expressed 37.1 kDa His-fusion protein as well as with Map sonicate.
The protein elicited significant delayed type hypersensitivity (DTH) skin reaction in mice sensitized with Map.
The results indicated that the recombinant PPE protein of Map was associated with cellular immune response.
American Psychological Association (APA)
Deb, Rajib& Goswami, P. P.. 2010. Expression of a Gene Encoding 34.9 kDa PPE Antigen of Mycobacterium avium subsp. paratuberculosis in E. coli. Molecular Biology International،Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-486353
Modern Language Association (MLA)
Deb, Rajib& Goswami, P. P.. Expression of a Gene Encoding 34.9 kDa PPE Antigen of Mycobacterium avium subsp. paratuberculosis in E. coli. Molecular Biology International No. 2010 (2010), pp.1-7.
https://search.emarefa.net/detail/BIM-486353
American Medical Association (AMA)
Deb, Rajib& Goswami, P. P.. Expression of a Gene Encoding 34.9 kDa PPE Antigen of Mycobacterium avium subsp. paratuberculosis in E. coli. Molecular Biology International. 2010. Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-486353
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-486353