Use of Bacterial Artificial Chromosomes in Baculovirus Research and Recombinant Protein Expression : Current Trends and Future Perspectives
Joint Authors
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-09-12
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The baculovirus expression system is one of the most successful and widely used eukaryotic protein expression methods.
This short review will summarise the role of bacterial artificial chromosomes (BACS) as an enabling technology for the modification of the virus genome.
For many years baculovirus genomes have been maintained in E.
coli as bacterial artificial chromosomes, and foreign genes have been inserted using a transposition-based system.
However, with recent advances in molecular biology techniques, particularly targeting reverse engineering of the baculovirus genome by recombineering, new frontiers in protein expression are being addressed.
In particular, BACs have facilitated the propagation of disabled virus genomes that allow high throughput protein expression.
Furthermore, improvement in the selection of recombinant viral genomes inserted into BACS has enabled the expression of multiprotein complexes by iterative recombineering of the baculovirus genome.
American Psychological Association (APA)
Roy, Polly& Noad, Rob. 2012. Use of Bacterial Artificial Chromosomes in Baculovirus Research and Recombinant Protein Expression : Current Trends and Future Perspectives. ISRN Microbiology،Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-486428
Modern Language Association (MLA)
Roy, Polly& Noad, Rob. Use of Bacterial Artificial Chromosomes in Baculovirus Research and Recombinant Protein Expression : Current Trends and Future Perspectives. ISRN Microbiology No. 2012 (2012), pp.1-11.
https://search.emarefa.net/detail/BIM-486428
American Medical Association (AMA)
Roy, Polly& Noad, Rob. Use of Bacterial Artificial Chromosomes in Baculovirus Research and Recombinant Protein Expression : Current Trends and Future Perspectives. ISRN Microbiology. 2012. Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-486428
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-486428