Xiao-Xu-Ming Decoction Protects against Blood-Brain Barrier Disruption and Neurological Injury Induced by Cerebral Ischemia and Reperfusion in Rats

Abstract EN

Xiao-Xu-Ming decoction (XXMD) is an effective prescription in the treatment of ischemic stroke, but the mechanisms involved are not well known.

In the present study, 120 male Sprague-Dawley rats were randomly divided into 5 groups: sham control (sham), ischemia and reperfusion (IR), and IR plus 15, 30, and 60 g/kg/day XXMD.

The stroke model was induced by 90 min of middle cerebral artery occlusion followed by reperfusion.

The brain lesion areas were evaluated by 2,3,5-triphenyltetrazolium chloride staining, and neurological deficits were observed at different time points after reperfusion.

Blood-brain barrier (BBB) disruption was evaluated by assessing brain water content and Evans blue content.

Pathological changes in BBB ultrastructure were observed with transmission electron microscopy.

MMP-9, -2, and VEGF expression levels were quantitatively determined by western blotting and immunohistochemistry.

We found that XXMD (60 g/kg/day) treatment reduced cerebral infarct area, improved behavioral function, and attenuated ultrastructure damage and permeability of BBB following ischemia and reperfusion.

Moreover, XXMD downregulated the expression levels of MMP-9, -2, and VEGF.

These findings indicate that XXMD alleviates BBB disruption and cerebral ischemic injury, which may be achieved by inhibiting the expression of MMP-9, -2, and VEGF.Erratum to “Xiao-Xu-Ming Decoction Protects against Blood-Brain Barrier Disruption and Neurological Injury Induced by Cerebral Ischemia and Reperfusion in Rats”dx.doi.org/10.1155/2013/476749