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Study on Genetic Variance of miR-541 in Type 1 Diabetes
Joint Authors
Han, Bei
Shi, Xing
Peng, Quan
Gao, Wentao
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-11
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Genetic susceptibility plays a key role in type 1 diabetes development.
Because miR-541 gene was located within the associated chromosome loci and its target genes include the diabetes-associated gene neurogenin3, this study aimed to investigate whether miR-541 had type 1 diabetes-associated genetic variations.
Type 1 diabetes children and healthy volunteers were recruited; direct sequencing was performed in initial 69 patients and 46 volunteers.
We identified 1 reported SNP (rs12893725) and 3 novel genetic variations, for the candidate -404 G→T variation, restriction fragment length polymorphism (RFLP) was performed in total 247 diabetes children and 212 healthy volunteers, a different distribution trait of allele frequencies was found between the two groups, and further clinical analysis found no significant correlation between clinical parameter and genotypes among patients.
In addition, by luciferase reporter assay, -404 was found to be within putative promoter region of pre-miR-541; although mutation of G→T has no effect on promoter activity, a significant secondary structure alteration may possibly influence its processing and transcription.
In conclusion, we identified 3 novel genetic variations in putative promoter of miR-541 in type 1 diabetes patients; -404 G→T of miR-541 is a potential T1D-associated genetic variation.
American Psychological Association (APA)
Han, Bei& Shi, Xing& Peng, Quan& Gao, Wentao. 2012. Study on Genetic Variance of miR-541 in Type 1 Diabetes. ISRN Endocrinology،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-486604
Modern Language Association (MLA)
Han, Bei…[et al.]. Study on Genetic Variance of miR-541 in Type 1 Diabetes. ISRN Endocrinology No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-486604
American Medical Association (AMA)
Han, Bei& Shi, Xing& Peng, Quan& Gao, Wentao. Study on Genetic Variance of miR-541 in Type 1 Diabetes. ISRN Endocrinology. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-486604
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-486604