Murine Pancreatic Beta TC3 Cells Show Greater 2′,5′-Oligoadenylate Synthetase (2′5′AS) Antiviral Enzyme Activity and Apoptosis Following IFN-α or Poly(I : C) Treatment than Pancreatic Alpha TC3 Cells
Joint Authors
Zheng, D.-J.
Li, M.
Bonnevie-Nielsen, V.
Field, L. L.
Source
Experimental Diabetes Research
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-10-29
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Type 1 diabetes is caused by autoimmune destruction of pancreatic beta cells, possibly virus initiated.
Virus infection induces alpha-interferon (IFN-α), leading to upregulation of genes encoding double-stranded (ds) RNA-dependent antiviral enzymes 2′,5′-oligoadenylate synthetase (2′5′AS) and PKR (p68).
To investigate whether beta cell specificity could be due to antiviral differences between beta and alpha cells, we treated beta and alpha TC3 cell lines with IFN-α and/or poly(I:C) (a synthetic dsRNA).
Results showed that, following IFN-α stimulation, increases in 2′5′AS levels and activities were significantly higher in beta than alpha cells (P<.001), whereas increases in PKR level and activity were comparable in the two cell types.
Poly(I:C) stimulated 2′5′AS activity in beta but not alpha cells, and co-transfection IFN-α plus poly(I:C) induced apoptosis in beta but not alpha cells.
These findings suggest that the elevated 2′5′AS response of pancreatic beta cells could render them particularly vulnerable to damage and/or apoptosis during virus infection.
American Psychological Association (APA)
Li, M.& Zheng, D.-J.& Field, L. L.& Bonnevie-Nielsen, V.. 2009. Murine Pancreatic Beta TC3 Cells Show Greater 2′,5′-Oligoadenylate Synthetase (2′5′AS) Antiviral Enzyme Activity and Apoptosis Following IFN-α or Poly(I : C) Treatment than Pancreatic Alpha TC3 Cells. Experimental Diabetes Research،Vol. 2009, no. 2009, pp.1-12.
https://search.emarefa.net/detail/BIM-486616
Modern Language Association (MLA)
Li, M.…[et al.]. Murine Pancreatic Beta TC3 Cells Show Greater 2′,5′-Oligoadenylate Synthetase (2′5′AS) Antiviral Enzyme Activity and Apoptosis Following IFN-α or Poly(I : C) Treatment than Pancreatic Alpha TC3 Cells. Experimental Diabetes Research No. 2009 (2009), pp.1-12.
https://search.emarefa.net/detail/BIM-486616
American Medical Association (AMA)
Li, M.& Zheng, D.-J.& Field, L. L.& Bonnevie-Nielsen, V.. Murine Pancreatic Beta TC3 Cells Show Greater 2′,5′-Oligoadenylate Synthetase (2′5′AS) Antiviral Enzyme Activity and Apoptosis Following IFN-α or Poly(I : C) Treatment than Pancreatic Alpha TC3 Cells. Experimental Diabetes Research. 2009. Vol. 2009, no. 2009, pp.1-12.
https://search.emarefa.net/detail/BIM-486616
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-486616