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Kinetic Approaches to Understanding the Mechanisms of Fidelity of the Herpes Simplex Virus Type 1 DNA Polymerase
Joint Authors
Parris, Deborah S.
Zhu, Yali
Stroud, Jason
Song, Liping
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-12-13
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
We discuss how the results of presteady-state and steady-state kinetic analysis of the polymerizing and excision activities of herpes simplex virus type 1 (HSV-1) DNA polymerase have led to a better understanding of the mechanisms controlling fidelity of this important model replication polymerase.
Despite a poorer misincorporation frequency compared to other replicative polymerases with intrinsic 3′ to 5′ exonuclease (exo) activity, HSV-1 DNA replication fidelity is enhanced by a high kinetic barrier to extending a primer/template containing a mismatch or abasic lesion and by the dynamic ability of the polymerase to switch the primer terminus between the exo and polymerizing active sites.
The HSV-1 polymerase with a catalytically inactivated exo activity possesses reduced rates of primer switching and fails to support productive replication, suggesting a novel means to target polymerase for replication inhibition.
American Psychological Association (APA)
Zhu, Yali& Stroud, Jason& Song, Liping& Parris, Deborah S.. 2010. Kinetic Approaches to Understanding the Mechanisms of Fidelity of the Herpes Simplex Virus Type 1 DNA Polymerase. Journal of Nucleic Acids،Vol. 2010, no. 2010, pp.1-15.
https://search.emarefa.net/detail/BIM-486666
Modern Language Association (MLA)
Zhu, Yali…[et al.]. Kinetic Approaches to Understanding the Mechanisms of Fidelity of the Herpes Simplex Virus Type 1 DNA Polymerase. Journal of Nucleic Acids No. 2010 (2010), pp.1-15.
https://search.emarefa.net/detail/BIM-486666
American Medical Association (AMA)
Zhu, Yali& Stroud, Jason& Song, Liping& Parris, Deborah S.. Kinetic Approaches to Understanding the Mechanisms of Fidelity of the Herpes Simplex Virus Type 1 DNA Polymerase. Journal of Nucleic Acids. 2010. Vol. 2010, no. 2010, pp.1-15.
https://search.emarefa.net/detail/BIM-486666
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-486666