Chemical Assistance in Refolding of Bacterial Inclusion Bodies
Joint Authors
Alibolandi, Mona
Mirzahoseini, Hasan
Source
Biochemistry Research International
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-08-01
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Escherichia coli is one of the most widely used hosts for the production of recombinant proteins but insoluble expression of heterologous proteins is a major bottleneck in production of recombinant proteins in E.
coli.
In vitro refolding of inclusion body into proteins with native conformations is a solution for this problem but there is a need for optimization of condition for each protein specifically.
Several approaches have been described for in vitro refolding; most of them involve the use of additives for assisting correct folding.
Cosolutes play a major role in refolding process and can be classified according to their function as aggregation suppressors and folding enhancers.
This paper presents a review of additives that are used in refolding process of insoluble recombinant proteins in small scale and industrial processes.
American Psychological Association (APA)
Alibolandi, Mona& Mirzahoseini, Hasan. 2011. Chemical Assistance in Refolding of Bacterial Inclusion Bodies. Biochemistry Research International،Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-486668
Modern Language Association (MLA)
Alibolandi, Mona& Mirzahoseini, Hasan. Chemical Assistance in Refolding of Bacterial Inclusion Bodies. Biochemistry Research International No. 2011 (2011), pp.1-6.
https://search.emarefa.net/detail/BIM-486668
American Medical Association (AMA)
Alibolandi, Mona& Mirzahoseini, Hasan. Chemical Assistance in Refolding of Bacterial Inclusion Bodies. Biochemistry Research International. 2011. Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-486668
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-486668