Purification of an Exopolygalacturonase from Penicillium viridicatum RFC3 Produced in Submerged Fermentation
Joint Authors
Gomes, Eleni
Leite, Rodrigo Simões Ribeiro
Silva, Dênis
da Silva, Roberto
Source
International Journal of Microbiology
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-02-03
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Abstract EN
An exo-PG obtained from Penicillium viridicatum in submerged fermentation was purified to homogeneity.
The apparent molecular weight of the enzyme was 92 kDa, optimum pH and temperature for activity were pH 5 and 50–55∘C.
The exo-PG showed a profile of an exo-polygalacturonase, releasing galacturonic acid by hydrolysis of pectin with a high degree of esterification (D.E.).
Ions Ca2+ enhanced the stability of enzyme and its activity by 30%.
The Km was 1.30 in absence of Ca2+ and 1.16 mg mL−1 in presence of this ion.
In relation to the Vmax the presence of this ion increased from 1.76 to 2.07 μmol min−1mg−1.
American Psychological Association (APA)
Gomes, Eleni& Leite, Rodrigo Simões Ribeiro& da Silva, Roberto& Silva, Dênis. 2010. Purification of an Exopolygalacturonase from Penicillium viridicatum RFC3 Produced in Submerged Fermentation. International Journal of Microbiology،Vol. 2009, no. 2009, pp.1-8.
https://search.emarefa.net/detail/BIM-486703
Modern Language Association (MLA)
Gomes, Eleni…[et al.]. Purification of an Exopolygalacturonase from Penicillium viridicatum RFC3 Produced in Submerged Fermentation. International Journal of Microbiology No. 2009 (2009), pp.1-8.
https://search.emarefa.net/detail/BIM-486703
American Medical Association (AMA)
Gomes, Eleni& Leite, Rodrigo Simões Ribeiro& da Silva, Roberto& Silva, Dênis. Purification of an Exopolygalacturonase from Penicillium viridicatum RFC3 Produced in Submerged Fermentation. International Journal of Microbiology. 2010. Vol. 2009, no. 2009, pp.1-8.
https://search.emarefa.net/detail/BIM-486703
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-486703
