Cell Wall Growth and Modulation Dynamics in a Model Unicellular Green Alga—Penium margaritaceum : Live Cell Labeling with Monoclonal Antibodies
Joint Authors
Britton, Alicia
Brechka, Hannah
Domozych, David S.
Toso, Marc
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-02-16
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Penium margaritaceum is a unicellular charophycean green alga that possesses cell wall polymers similar to those of land plants.
Several wall macromolecules of this alga are recognized by monoclonal antibodies specific for wall polymer epitopes of land plants.
Immunofluorescence protocols using these antibodies may be employed to label specific cell wall constituents of live cells.
Fluorescent labeling persists for several days, and this attribute allows for tracing of wall epitopes in both long- and short-term studies of cell development.
Quantitative analysis of surface area covered by cell wall polymers is also easily performed.
We show that significant cell expansion caused by incubation of cells in low levels of osmotically active agents like mannitol, glucose, or sucrose results from the inability of cells to undergo cytokinesis but does not result in significant changes to the amount of new cell wall.
We also demonstrate that cells can be maintained for long periods of time in culture medium supplemented with specific cell wall-degrading enzymes where notable changes to wall infrastructure occur.
These results demonstrate the great potential value of Penium in elucidating fundamental events during cell wall synthesis and modulation in plant cells.
American Psychological Association (APA)
Domozych, David S.& Brechka, Hannah& Britton, Alicia& Toso, Marc. 2011. Cell Wall Growth and Modulation Dynamics in a Model Unicellular Green Alga—Penium margaritaceum : Live Cell Labeling with Monoclonal Antibodies. Journal of Botany،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-486727
Modern Language Association (MLA)
Domozych, David S.…[et al.]. Cell Wall Growth and Modulation Dynamics in a Model Unicellular Green Alga—Penium margaritaceum : Live Cell Labeling with Monoclonal Antibodies. Journal of Botany No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-486727
American Medical Association (AMA)
Domozych, David S.& Brechka, Hannah& Britton, Alicia& Toso, Marc. Cell Wall Growth and Modulation Dynamics in a Model Unicellular Green Alga—Penium margaritaceum : Live Cell Labeling with Monoclonal Antibodies. Journal of Botany. 2011. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-486727
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-486727