Electrophoretic Mobility of Cardiac Myosin Heavy Chain Isoforms Revisited : Application of MALDI TOFTOF Analysis
Joint Authors
Soukup, Tomáš
Arnostova, Petra
Jedelsky, Petr L.
Zurmanova, Jitka
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-11-30
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The expression of two cardiac myosin heavy chain (MyHC) isoforms in response to the thyroid status was studied in left ventricles (LVs) of Lewis rats.
Major MyHC isoform in euthyroid and hyperthyroid LVs had a higher mobility on SDS-PAGE, whereas hypothyroid LVs predominantly contained a MyHC isoform with a lower mobility corresponding to that of the control soleus muscle.
By comparing the MyHC profiles obtained under altered thyroid states together with the control soleus, we concluded that MyHCα was represented by the lower band with higher mobility and MyHCβ by the upper band.
The identity of these two bands in SDS-PAGE gels was confirmed by western blot and mass spectrometry.
Thus, in contrast to the literature data, we found that the MyHCα possessed a higher mobility rate than the MyHCβ isoform.
Our data highlighted the importance of the careful identification of the MyHCα and MyHCβ isoforms analyzed by the SDS-PAGE.
American Psychological Association (APA)
Arnostova, Petra& Jedelsky, Petr L.& Soukup, Tomáš& Zurmanova, Jitka. 2011. Electrophoretic Mobility of Cardiac Myosin Heavy Chain Isoforms Revisited : Application of MALDI TOFTOF Analysis. Journal of Biomedicine and Biotechnology،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-486841
Modern Language Association (MLA)
Arnostova, Petra…[et al.]. Electrophoretic Mobility of Cardiac Myosin Heavy Chain Isoforms Revisited : Application of MALDI TOFTOF Analysis. Journal of Biomedicine and Biotechnology No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-486841
American Medical Association (AMA)
Arnostova, Petra& Jedelsky, Petr L.& Soukup, Tomáš& Zurmanova, Jitka. Electrophoretic Mobility of Cardiac Myosin Heavy Chain Isoforms Revisited : Application of MALDI TOFTOF Analysis. Journal of Biomedicine and Biotechnology. 2011. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-486841
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-486841