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Mass Spectrometric Screening of Ovarian Cancer with Serum Glycans
Joint Authors
Kim, Tae Jin
Jo, Yohahn
Um, Dalho
An, Hyunjoo
Kim, Jae-Han
Kim, Yangsun
Park, Chang Won
Baek, Ki Hwang
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-04
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Changes of glycosylation pattern in serum proteins have been linked to various diseases including cancer, suggesting possible development of novel biomarkers based on the glycomic analysis.
In this study, N-linked glycans from human serum were quantitatively profiled by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) and compared between healthy controls and ovarian cancer patients.
A training set consisting of 40 healthy controls and 40 ovarian cancer cases demonstrated an inverse correlation between P value of ANOVA and area under the curve (AUC) of each candidate biomarker peak from MALDI-TOF MS, providing standards for the classification.
A multibiomarker panel composed of 15 MALDI-TOF MS peaks resulted in AUC of 0.89, 80~90% sensitivity, and 70~83% specificity in the training set.
The performance of the biomarker panel was validated in a separate blind test set composed of 23 healthy controls and 37 ovarian cancer patients, leading to 81~84% sensitivity and 83% specificity with cut-off values determined by the training set.
Sensitivity of CA-125, the most widely used ovarian cancer marker, was 74% in the training set and 78% in the test set, respectively.
These results indicate that MALDI-TOF MS-mediated serum N-glycan analysis could provide critical information for the screening of ovarian cancer.
American Psychological Association (APA)
Kim, Jae-Han& Park, Chang Won& Um, Dalho& Baek, Ki Hwang& Jo, Yohahn& An, Hyunjoo…[et al.]. 2014. Mass Spectrometric Screening of Ovarian Cancer with Serum Glycans. Disease Markers،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-486846
Modern Language Association (MLA)
Kim, Jae-Han…[et al.]. Mass Spectrometric Screening of Ovarian Cancer with Serum Glycans. Disease Markers No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-486846
American Medical Association (AMA)
Kim, Jae-Han& Park, Chang Won& Um, Dalho& Baek, Ki Hwang& Jo, Yohahn& An, Hyunjoo…[et al.]. Mass Spectrometric Screening of Ovarian Cancer with Serum Glycans. Disease Markers. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-486846
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-486846