Aldehyded Dextran and ε-Poly(L-lysine) Hydrogel as Nonviral Gene Carrier
Joint Authors
Bessho, Kazuhisa
Hyon, Suong-Hyu
Takahashi, Katsu
Togo, Yumiko
Tsukamoto, Hiroko
Kiso, Honoka
Saito, Kazuyuki
Huang, Boyen
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-08-21
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Abstract EN
Background.
The expression term of the gene transfected in cells needs to belong enough inorder to make a gene therapy clinically effective.
The controlled release of the transfected gene can be utilized.
The new biodegradable hydrogel material created by 20 w/w% aldehyded dextran and 10 w/w% ε-poly(L-lysine) (ald-dex/PLL) was developed.
We examined whether it could be as a nonviral carrier of the gene transfer.
Methods.
A plasmid (Lac-Z) was mixed with ald-dex/PLL.
An in vitro study was performed to assess the expression of Lac-Z with X-gal stain after gene transfer into the cultured 293 cells and bone marrow cells.
As a control group, PLL was used as a cationic polymer.
Results.
We confirmed that the transfection efficiency of the ald-dex/PLL had a higher transfection efficiency than PLL in 293 cells (plasmid of 2 μg: ald-dex/PLL 1.1%, PLL 0.23%, plasmid of 16 μg: ald-dex/PLL 1.23%, PLL 0.48%).
In bone marrow cells, we confirmed the expression of Lac-Z by changing the quantity of aldehyded dextran.
In the groups using ald-dextran of the quantity of 1/4 and 1/12 of PLL, their transfection efficiency was 0.43% and 0.41%, respectively.
Conclusions.
This study suggested a potential of using ald-dex/PLL as a non-carrier for gene transfer.
American Psychological Association (APA)
Togo, Yumiko& Takahashi, Katsu& Saito, Kazuyuki& Kiso, Honoka& Huang, Boyen& Tsukamoto, Hiroko…[et al.]. 2013. Aldehyded Dextran and ε-Poly(L-lysine) Hydrogel as Nonviral Gene Carrier. Stem Cells International،Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-486855
Modern Language Association (MLA)
Togo, Yumiko…[et al.]. Aldehyded Dextran and ε-Poly(L-lysine) Hydrogel as Nonviral Gene Carrier. Stem Cells International No. 2013 (2013), pp.1-5.
https://search.emarefa.net/detail/BIM-486855
American Medical Association (AMA)
Togo, Yumiko& Takahashi, Katsu& Saito, Kazuyuki& Kiso, Honoka& Huang, Boyen& Tsukamoto, Hiroko…[et al.]. Aldehyded Dextran and ε-Poly(L-lysine) Hydrogel as Nonviral Gene Carrier. Stem Cells International. 2013. Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-486855
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-486855