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Activation of PPARs α, βδ, and γ Impairs TGF-β1-Induced Collagens' Production and Modulates the TIMP-1MMPs Balance in Three-Dimensional Cultured Chondrocytes
Joint Authors
Poleni, Paul-Emile
Velot, Emilie
Netter, Patrick
Bianchi, Arnaud
Etienne, Stephanie
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-10-04
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Background and Purpose.
We investigated the potency of Peroxisome Proliferators-Activated Receptors (PPARs) α, β/δ, and γ agonists to modulate Transforming Growth Factor-β1 (TGF-β1-) induced collagen production or changes in Tissue Inhibitor of Matrix Metalloproteinase- (TIMP-) 1/Matrix Metalloproteinase (MMP) balance in rat chondrocytes embedded in alginate beads.
Experimental Approach.
Collagen production was evaluated by quantitative Sirius red staining, while TIMP-1 protein levels and global MMP (-1, -2, -3, -7, and -9) or specific MMP-13 activities were measured by ELISA and fluorigenic assays in culture media, respectively.
Levels of mRNA for type II collagen, TIMP-1, and MMP-3 & 13 were quantified by real-time PCR.
Key Results.
TGF-β1 increased collagen deposition and type II collagen mRNA levels, while inducing TIMP-1 mRNA and protein expression.
In contrast, it decreased global MMP or specific MMP-13 activities, while decreasing MMP-3 or MMP-13 mRNA levels.
PPAR agonists reduced most of the effects of TGF-β1 on changes in collagen metabolism and TIMP-1/MMP balance in rat in a PPAR-dependent manner, excepted for Wy14643 on MMP activities.
Conclusions and Implications.
PPAR agonists reduce TGF-β1-modulated ECM turnover and inhibit chondrocyte activities crucial for collagen biosynthesis, and display a different inhibitory profile depending on selectivity for PPAR isotypes.
American Psychological Association (APA)
Poleni, Paul-Emile& Etienne, Stephanie& Velot, Emilie& Netter, Patrick& Bianchi, Arnaud. 2010. Activation of PPARs α, βδ, and γ Impairs TGF-β1-Induced Collagens' Production and Modulates the TIMP-1MMPs Balance in Three-Dimensional Cultured Chondrocytes. PPAR Research،Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-487000
Modern Language Association (MLA)
Poleni, Paul-Emile…[et al.]. Activation of PPARs α, βδ, and γ Impairs TGF-β1-Induced Collagens' Production and Modulates the TIMP-1MMPs Balance in Three-Dimensional Cultured Chondrocytes. PPAR Research No. 2010 (2010), pp.1-11.
https://search.emarefa.net/detail/BIM-487000
American Medical Association (AMA)
Poleni, Paul-Emile& Etienne, Stephanie& Velot, Emilie& Netter, Patrick& Bianchi, Arnaud. Activation of PPARs α, βδ, and γ Impairs TGF-β1-Induced Collagens' Production and Modulates the TIMP-1MMPs Balance in Three-Dimensional Cultured Chondrocytes. PPAR Research. 2010. Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-487000
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-487000