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Xanthan Gum as an Adjuvant in a Subunit Vaccine Preparation against Leptospirosis
Joint Authors
Hartwig, Daiane D.
McBride, Alan J. A.
Bacelo, Katia L.
Vendrusculo, Claire T.
Seixas, Fabiana K.
Collares, Tiago
Amaral, Marta
Schuch, Rodrigo
Dellagostin, Odir A.
Moreira, Angelita da S.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-07
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Leptospiral immunoglobulin-like (Lig) proteins are of great interest due to their ability to act as mediators of pathogenesis, serodiagnostic antigens, and immunogens.
Purified recombinant LigA protein is the most promising subunit vaccine candidate against leptospirosis reported to date, however, as purified proteins are weak immunogens the use of a potent adjuvant is essential for the success of LigA as a subunit vaccine.
In the present study, we compared xanthan pv.
pruni (strain 106), aluminium hydroxide (alhydrogel), and CpG ODN as adjuvants in a LigA subunit vaccine preparation.
Xanthan gum is a high molecular weight extracellular polysaccharide produced by fermentation of Xanthomonas spp., a plant-pathogenic bacterium genus.
Preparations containing xanthan induced a strong antibody response comparable to that observed when alhydrogel was used.
Upon challenge with a virulent strain of L.
interrogans serovar Copenhageni, significant protection (Fisher test, P<0.05) was observed in 100%, 100%, and 67% of hamsters immunized with rLigANI-xanthan, LigA-CpG-xanthan, and rLigANI-alhydrogel, respectively.
Furthermore, xanthan did not cause cytotoxicity in Chinese hamster ovary (CHO) cells in vitro.
The use of xanthan as an adjuvant is a novel alternative for enhancing the immunogenicity of vaccines against leptospirosis and possibly against other pathogens.
American Psychological Association (APA)
Bacelo, Katia L.& Hartwig, Daiane D.& Seixas, Fabiana K.& Schuch, Rodrigo& Moreira, Angelita da S.& Amaral, Marta…[et al.]. 2014. Xanthan Gum as an Adjuvant in a Subunit Vaccine Preparation against Leptospirosis. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-487050
Modern Language Association (MLA)
Bacelo, Katia L.…[et al.]. Xanthan Gum as an Adjuvant in a Subunit Vaccine Preparation against Leptospirosis. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-487050
American Medical Association (AMA)
Bacelo, Katia L.& Hartwig, Daiane D.& Seixas, Fabiana K.& Schuch, Rodrigo& Moreira, Angelita da S.& Amaral, Marta…[et al.]. Xanthan Gum as an Adjuvant in a Subunit Vaccine Preparation against Leptospirosis. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-487050
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-487050