Transcription Factor Sp1 Is Involved in Expressional Regulation of Coxsackie and Adenovirus Receptor in Cancer Cells
Joint Authors
Lim, Joong-Yeon
Nam, Jae-Hwan
Hwang, Ha-Young
Kim, Joo-Young
Park, Sang Ick
Park, Young Mi
Chung, Sun-Ku
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-11-24
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Coxsackie and adenovirus receptor (CAR) was first known as a virus receptor.
Recently, it is also known to have tumor suppressive activity such as inhibition of cell proliferation, migration, and invasion.
It is important to understand how CAR expression can be regulated in cancers.
Based on an existence of putative Sp1 binding site within CAR promoter, we investigated whether indeed Sp1 is involved in the regulation of CAR expression.
We observed that deletion or mutation of Sp1 binding motif (−503/−498) prominently impaired the Sp1 binding affinity and activity of CAR promoter.
Histone deacetylase inhibitor (TSA) treatment enhanced recruitment of Sp1 to the CAR promoter in ChIP assay.
Meanwhile, Sp1 binding inhibitor suppressed the recruitment.
Exogenous expression of wild-type Sp1 increased CAR expression in CAR-negative cells; meanwhile, dominant negative Sp1 decreased the CAR expression in CAR-positive cells.
These results indicate that Sp1 is involved in regulation of CAR expression.
American Psychological Association (APA)
Chung, Sun-Ku& Kim, Joo-Young& Lim, Joong-Yeon& Park, Young Mi& Hwang, Ha-Young& Nam, Jae-Hwan…[et al.]. 2011. Transcription Factor Sp1 Is Involved in Expressional Regulation of Coxsackie and Adenovirus Receptor in Cancer Cells. Journal of Biomedicine and Biotechnology،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-487053
Modern Language Association (MLA)
Chung, Sun-Ku…[et al.]. Transcription Factor Sp1 Is Involved in Expressional Regulation of Coxsackie and Adenovirus Receptor in Cancer Cells. Journal of Biomedicine and Biotechnology No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-487053
American Medical Association (AMA)
Chung, Sun-Ku& Kim, Joo-Young& Lim, Joong-Yeon& Park, Young Mi& Hwang, Ha-Young& Nam, Jae-Hwan…[et al.]. Transcription Factor Sp1 Is Involved in Expressional Regulation of Coxsackie and Adenovirus Receptor in Cancer Cells. Journal of Biomedicine and Biotechnology. 2011. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-487053
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-487053