Repetitive Peroxide Exposure Reveals Pleiotropic Mitogen-Activated Protein Kinase Signaling Mechanisms
Joint Authors
Wang, Liyun
Brenneman, Randall
Chadwick, Wayne
Park, Sung-Soo
Maudsley, Stuart
Zhou, Yu
Martin, Bronwen
Keselman, Alex
Source
Journal of Signal Transduction
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-12-19
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Oxidative stressors such as hydrogen peroxide control the activation of many interconnected signaling systems and are implicated in neurodegenerative disease etiology.
Application of hydrogen peroxide to PC12 cells activated multiple tyrosine kinases (c-Src, epidermal growth factor receptor (EGFR), and Pyk2) and the serine-threonine kinase ERK1/2.
Peroxide-induced ERK1/2 activation was sensitive to intracellular calcium chelation and EGFR and c-Src kinase inhibition.
Acute application and removal of peroxide allowed ERK1/2 activity levels to rapidly subside to basal serum-deprived levels.
Using this protocol, we demonstrated that ERK1/2 activation tachyphylaxis developed upon repeated peroxide exposures.
This tachyphylaxis was independent of c-Src/Pyk2 tyrosine phosphorylation but was associated with a progressive reduction of peroxide-induced EGFR tyrosine phosphorylation, EGFR interaction with growth factor receptor binding protein 2, and a redistribution of EGFR from the plasma membrane to the cytoplasm.
Our data indicates that components of peroxide-induced ERK1/2 cascades are differentially affected by repeated exposures, indicating that oxidative signaling may be contextually variable.
American Psychological Association (APA)
Chadwick, Wayne& Keselman, Alex& Park, Sung-Soo& Zhou, Yu& Wang, Liyun& Brenneman, Randall…[et al.]. 2010. Repetitive Peroxide Exposure Reveals Pleiotropic Mitogen-Activated Protein Kinase Signaling Mechanisms. Journal of Signal Transduction،Vol. 2011, no. 2011, pp.1-15.
https://search.emarefa.net/detail/BIM-487089
Modern Language Association (MLA)
Chadwick, Wayne…[et al.]. Repetitive Peroxide Exposure Reveals Pleiotropic Mitogen-Activated Protein Kinase Signaling Mechanisms. Journal of Signal Transduction No. 2011 (2011), pp.1-15.
https://search.emarefa.net/detail/BIM-487089
American Medical Association (AMA)
Chadwick, Wayne& Keselman, Alex& Park, Sung-Soo& Zhou, Yu& Wang, Liyun& Brenneman, Randall…[et al.]. Repetitive Peroxide Exposure Reveals Pleiotropic Mitogen-Activated Protein Kinase Signaling Mechanisms. Journal of Signal Transduction. 2010. Vol. 2011, no. 2011, pp.1-15.
https://search.emarefa.net/detail/BIM-487089
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-487089