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Effect of Thickness of HA-Coating on Microporous Silk Scaffolds Using Alternate Soaking Technology
Joint Authors
Li, Hongguo
Xie, Zhenghong
Fan, Xiangli
Hao, Zhangying
Zhu, Rui
Xue, Yingsen
Sun, Liguo
Fan, Hongbin
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-28
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Hydroxyapatite (HA) can be coated on various materials surface and has the function of osteogenicity.
Microporous silk scaffold has excellent biocompatibility.
In this study, alternate soaking technology was used to coat HA on microporous silk scaffolds.
However, the cell proliferation was found to decrease with the increasing thickness (cycles of soaking) of HA-coating.
This study aims to determine the best thickness (cycles of soaking) of HA-coating on microporous silk scaffolds.
The SEM observation showed that group with one cycle of alternate soaking (1C-HA) has the most optimal porosity like non-HA-modified microporous silk scaffolds.
The proliferation of osteoblasts has no significant difference between noncoated HA (N-HA) and 1C-HA groups, which are both significantly higher than those in two cycles of soaking (2C-HA) and three cycles of soaking (3C-HA) groups.
The transcription levels of specific genes (runx2 and osteonectin) in osteoblasts of 1C-HA group were significantly higher than those of N-HA group.
Moreover, the levels showed no significant difference among 1C-HA, 2C-HA, and 3C-HA groups.
In conclusion, microporous silk scaffold with 1 cycle of HA-coating can combine the biocompatibility of silk and osteogenicity of HA.
American Psychological Association (APA)
Li, Hongguo& Zhu, Rui& Sun, Liguo& Xue, Yingsen& Hao, Zhangying& Xie, Zhenghong…[et al.]. 2014. Effect of Thickness of HA-Coating on Microporous Silk Scaffolds Using Alternate Soaking Technology. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-487162
Modern Language Association (MLA)
Li, Hongguo…[et al.]. Effect of Thickness of HA-Coating on Microporous Silk Scaffolds Using Alternate Soaking Technology. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-487162
American Medical Association (AMA)
Li, Hongguo& Zhu, Rui& Sun, Liguo& Xue, Yingsen& Hao, Zhangying& Xie, Zhenghong…[et al.]. Effect of Thickness of HA-Coating on Microporous Silk Scaffolds Using Alternate Soaking Technology. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-487162
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-487162