Assessment of Pseudoaffinity Chromatography Using Textile Dyes for Isolation of Buffalo Pituitary Luteinizing Hormone
Joint Authors
Patel, Pankaj
Arora, Taruna
Muralidhar, K.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-16
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Extensive investigation has been carried out to elucidate the mechanisms involved in pseudoligand affinity chromatography using textile dyes, and, empirically, it has been attributed to the chemical and steric structures of dye and protein.
Possibly, a variety of interactions especially ionic and/or hydrophobic influence with a varying share in the binding and differ from protein to protein and from dye to dye.
In this study, we have attempted to understand the effect of various biophysical parameters like the nature of the eluant, pH, and ionic strength on the binding of crude luteinizing hormone (LH) with various triazine-based dyes and thus predict their nature.
Based on the elution patterns, cibacron and reactive brown suggested a dual electrostatic and hydrophobic nature.
Reactive blue and reactive yellow reflected a major electrostatic/ionic nature with yellow offering 50-fold purification in a single step, while reactive red and reactive green had a predominant hydrophobic nature.
Appreciably, reactive red was binding LH very tightly unlike other dyes, and addition of the arginine in the elution buffer substantially weakened the protein-dye interactions.
pH was observed to be a principal factor assisting the protein-dye binding as well as hydrophobicity of the dye and the proteins.
American Psychological Association (APA)
Arora, Taruna& Patel, Pankaj& Muralidhar, K.. 2012. Assessment of Pseudoaffinity Chromatography Using Textile Dyes for Isolation of Buffalo Pituitary Luteinizing Hormone. ISRN Chromatography،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-487345
Modern Language Association (MLA)
Arora, Taruna…[et al.]. Assessment of Pseudoaffinity Chromatography Using Textile Dyes for Isolation of Buffalo Pituitary Luteinizing Hormone. ISRN Chromatography No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-487345
American Medical Association (AMA)
Arora, Taruna& Patel, Pankaj& Muralidhar, K.. Assessment of Pseudoaffinity Chromatography Using Textile Dyes for Isolation of Buffalo Pituitary Luteinizing Hormone. ISRN Chromatography. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-487345
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-487345