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Detection of Genetic Variations in Coagulopathy-Related Genes Using Ramified Rolling Circle Amplification
Joint Authors
Smith, James H.
Ye, Fei
Cui, Miao
Beals, Thomas P.
Zhang, David Y.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-01
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
We evaluated single nucleotide polymorphism (SNP) detection via a target-capture, C-probe ligation, and RAM assay in a single-blind comparison to clinical samples that had been tested with FDA-cleared tests for up to 4 different vascular disease-related SNPs.
In the RAM assay circulizable linear probes (C- or padlock probes) were annealed directly to genomic DNA, processed on a largely automated platform, and ligated C-probes were amplified by real-time RAM.
After allele determinations were made with the experimental system, the sample genotypes were unblinded and the experimentally determined genotypes were found to be completely consistent with the FDA-cleared test results.
The methods and results presented here show that a combination of C-probes, automated sample processing, and isothermal RAM provides a robust, and specific, nucleic acid detection platform that is compatible with automated DNA sample preparation and the throughput requirements of the clinical laboratory.
American Psychological Association (APA)
Smith, James H.& Cui, Miao& Zhang, David Y.& Beals, Thomas P.& Ye, Fei. 2014. Detection of Genetic Variations in Coagulopathy-Related Genes Using Ramified Rolling Circle Amplification. BioMed Research International،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-487490
Modern Language Association (MLA)
Smith, James H.…[et al.]. Detection of Genetic Variations in Coagulopathy-Related Genes Using Ramified Rolling Circle Amplification. BioMed Research International No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-487490
American Medical Association (AMA)
Smith, James H.& Cui, Miao& Zhang, David Y.& Beals, Thomas P.& Ye, Fei. Detection of Genetic Variations in Coagulopathy-Related Genes Using Ramified Rolling Circle Amplification. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-487490
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-487490