Upregulation of TLR1, TLR2, TLR4, and IRAK-2 Expression During ML-1 Cell Differentiation to Macrophages : Role in the Potentiation of Cellular Responses to LPS and LTA
Joint Authors
Traore, Kassim
Thimmulappa, Rajesh K.
Biswal, Shyam
Trush, Michael A.
Zirkin, Barry
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-21
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
12-O-tetradecanoylphorbol 13-acetate (TPA) induces the differentiation of human myeloid ML-1 cells to macrophages.
In the current study, the expression, responsiveness, and regulation of toll-like receptors (TLRs) in TPA-induced ML-1-derived macrophages were investigated.
We have found that TPA-induced differentiation of ML-1 cells was accompanied by the upregulation of TLR1, TLR2, TLR4, and CD14 expression at both the mRNA and protein levels.
Interestingly, TLR1 and TLR4 protein expression on ML-1 cells could be blocked by pretreatment with U0126, suggesting the role of an Erk1/2-induced differentiation signal in this process.
In addition, the expression of IRAK-2, a key member of the TLR/IRAK-2/NF-κB-dependent signaling cascade was also induced in response to TPA.
Accordingly, we demonstrated an increased cellular release of inflammatory cytokines (TNF-α and various interleukins) upon stimulation with LPS and LTA ligands for TLR4 and TLR2, respectively.
Furthermore, using luminol-dependent chemiluminescence, addition of LPS and LTA induces a sustained DPI-inhibitable generation of reactive oxygen species (ROS) by the differentiated ML-1 cells.
Together, these data suggest that the increase in the responsiveness of TPA-treated ML-1 cells to LPS and LTA occurs in response to the upregulation of their respective receptors as well as an induction of the IRAK-2 gene expression.
American Psychological Association (APA)
Traore, Kassim& Zirkin, Barry& Thimmulappa, Rajesh K.& Biswal, Shyam& Trush, Michael A.. 2012. Upregulation of TLR1, TLR2, TLR4, and IRAK-2 Expression During ML-1 Cell Differentiation to Macrophages : Role in the Potentiation of Cellular Responses to LPS and LTA. ISRN Oncology،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-487496
Modern Language Association (MLA)
Traore, Kassim…[et al.]. Upregulation of TLR1, TLR2, TLR4, and IRAK-2 Expression During ML-1 Cell Differentiation to Macrophages : Role in the Potentiation of Cellular Responses to LPS and LTA. ISRN Oncology No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-487496
American Medical Association (AMA)
Traore, Kassim& Zirkin, Barry& Thimmulappa, Rajesh K.& Biswal, Shyam& Trush, Michael A.. Upregulation of TLR1, TLR2, TLR4, and IRAK-2 Expression During ML-1 Cell Differentiation to Macrophages : Role in the Potentiation of Cellular Responses to LPS and LTA. ISRN Oncology. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-487496
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-487496