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Screening of Supports for the Immobilization of β-Glucosidase
Joint Authors
Sato, Hélia Harumi
Fernandes, Pedro
Dias, Fernanda Furlan Gonçalves
Figueira, Joelise de Alencar
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-09-11
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
A set of supports were screened for the immobilization of a partially purified extract of β-glucosidase from Aspergillus sp.
These supports, namely, Eupergit, Amberlite, alginate, gelatin, polyvinyl alcohol- (PVA-) based matrices (Lentikats), and sol-gel, have proved effective for the implementation of some other enzyme-based processes.
The initial criterion for selection of promising supports prior to further characterization relied on the retention of the catalytic activity following immobilization.
Based on such criterion, where immobilization in sol-gel and in Lentikats outmatched the remaining approaches, those two systems were further characterized.
Immobilization did not alter the pH/activity profile, whereas the temperature/activity profile was improved when sol-gel support was assayed.
Both thermal and pH stability were improved as a result of immobilization.
An increase in the apparent KM (Michaelis constant) was observed following immobilization, suggesting diffusion limitations.
American Psychological Association (APA)
Figueira, Joelise de Alencar& Dias, Fernanda Furlan Gonçalves& Sato, Hélia Harumi& Fernandes, Pedro. 2011. Screening of Supports for the Immobilization of β-Glucosidase. Enzyme Research،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-487592
Modern Language Association (MLA)
Figueira, Joelise de Alencar…[et al.]. Screening of Supports for the Immobilization of β-Glucosidase. Enzyme Research No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-487592
American Medical Association (AMA)
Figueira, Joelise de Alencar& Dias, Fernanda Furlan Gonçalves& Sato, Hélia Harumi& Fernandes, Pedro. Screening of Supports for the Immobilization of β-Glucosidase. Enzyme Research. 2011. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-487592
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-487592