![](/images/graphics-bg.png)
Chemical Separation of Fixed Tissue Using Thermolysin
Joint Authors
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-31
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Thermolysin is a metallopeptidase used to cleave peptide bonds at specific junctions.
It has previously been used to cleave specific amino acid sequences found at the junction of the sensory epithelium and underlying stroma of unfixed otolithic organs of the vestibular system.
We have used thermolysin to separate sensory epithelium from the underlying stroma in fixed cristae ampullares of mouse, rat, gerbil, guinea pig, chinchilla, and tree squirrel, thus removing the saddle-shaped curvature of the sensory organ and creating a flattened sensory epithelium preparation.
This permits visualization of the entire sensory organ in a single mount and facilitates proper morphometric analysis.Erratum to “Chemical Separation of Fixed Tissue Using Thermolysin”dx.doi.org/10.1155/2014/729846
American Psychological Association (APA)
Dua, Anahita& Desai, Sapan S.. 2013. Chemical Separation of Fixed Tissue Using Thermolysin. Journal of Histology،Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-487696
Modern Language Association (MLA)
Dua, Anahita& Desai, Sapan S.. Chemical Separation of Fixed Tissue Using Thermolysin. Journal of Histology No. 2013 (2013), pp.1-5.
https://search.emarefa.net/detail/BIM-487696
American Medical Association (AMA)
Dua, Anahita& Desai, Sapan S.. Chemical Separation of Fixed Tissue Using Thermolysin. Journal of Histology. 2013. Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-487696
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-487696