Highly Sensitive High Performance Liquid Chromatography-Laser Induced Fluorescence for Proteomics Applications
Joint Authors
Prabhu, Vijendra
Chidangil, Santhosh
Choudhari, K. S.
Unnikrishnan, V. K.
Patil, Ajeetkumar
Bhat, Sujatha
Pai, Keerthilatha M.
Kartha, V. B.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-08-21
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
This paper describes the sensitivity study and performance evaluation of high-performance liquid chromatography-laser-induced fluorescence detection (HPLC-LIF) system assembled in our laboratory for proteomics applications.
The limits of Detection (LOD) of several serum proteins have been estimated with this instrument and are found to be much lower compared to other commonly used proteomics techniques like SELDI, MALDI, 2-D-SDS-PAGE, and so forth.
Techniques for improving the LOD still further with similar setup are briefly discussed.
Using the system, protein profiles of serum in normal, malignant, and premalignant conditions were recorded for different malignancy situations.
American Psychological Association (APA)
Patil, Ajeetkumar& Choudhari, K. S.& Prabhu, Vijendra& Unnikrishnan, V. K.& Bhat, Sujatha& Pai, Keerthilatha M.…[et al.]. 2012. Highly Sensitive High Performance Liquid Chromatography-Laser Induced Fluorescence for Proteomics Applications. ISRN Spectroscopy،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-487733
Modern Language Association (MLA)
Patil, Ajeetkumar…[et al.]. Highly Sensitive High Performance Liquid Chromatography-Laser Induced Fluorescence for Proteomics Applications. ISRN Spectroscopy No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-487733
American Medical Association (AMA)
Patil, Ajeetkumar& Choudhari, K. S.& Prabhu, Vijendra& Unnikrishnan, V. K.& Bhat, Sujatha& Pai, Keerthilatha M.…[et al.]. Highly Sensitive High Performance Liquid Chromatography-Laser Induced Fluorescence for Proteomics Applications. ISRN Spectroscopy. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-487733
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-487733