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Isolation of Resveratrol from Vitis Viniferae Caulis and Its Potent Inhibition of Human Tyrosinase
Joint Authors
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-02-12
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Tyrosinase (TYR) catalyzes rate-limiting reactions of cellular melanin synthesis, and its inhibitors are of commercial interest as potential skin whitening agents.
However, the limited availability of human TYR makes the screening of TYR inhibitors difficult.
To overcome this hurdle, we transformed nonmelanocytic human embryonic kidney (HEK) 293 cells to express human TYR constitutively.
Using these cells as a source of human TYR, the ethanolic extracts of 52 medicinal plants grown in Korea were tested for human TYR activity, and the extract of Vitis Viniferae Caulis (dried stems of the grape tree, Vitis vinifera L.) was found to inhibit human TYR activity potently.
An active compound was isolated from this extract by solvent fractionation followed by liquid column chromatography and identified as resveratrol by spectroscopic and chromatographic analyses.
Resveratrol was determined to be a highly potent inhibitor of human TYR (IC50=0.39 μg mL−1) as compared with p-coumaric acid (IC50=0.66 μg mL−1) and arbutin (IC50>100 μg mL−1) and inhibited melanin synthesis by human epidermal melanocytes at subtoxic concentrations.
This study suggests that resveratrol and resveratrol-containing extracts of Vitis Viniferae Caulis have a potential use as skin whitening agents.
American Psychological Association (APA)
Park, Jiaa& Boo, Yong Chool. 2013. Isolation of Resveratrol from Vitis Viniferae Caulis and Its Potent Inhibition of Human Tyrosinase. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-487767
Modern Language Association (MLA)
Park, Jiaa& Boo, Yong Chool. Isolation of Resveratrol from Vitis Viniferae Caulis and Its Potent Inhibition of Human Tyrosinase. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-487767
American Medical Association (AMA)
Park, Jiaa& Boo, Yong Chool. Isolation of Resveratrol from Vitis Viniferae Caulis and Its Potent Inhibition of Human Tyrosinase. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-487767
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-487767