An Improved Method to Knock Out the asd Gene of Salmonella enterica Serovar Pullorum
Joint Authors
Chen, Xiao-Juan
Pan, Zhi-ming
Jiao, Xin-an
Geng, Shi-Zhong
Zhang, Xiao-Ming
Chen, Xiang
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-08-09
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
An asd-deleted (Δasd) mutant of Salmonella enterica serovar Pullorum (SP) was constructed using an improved method of gene knockout by combining the π-suicide plasmid system with the Red Disruption system.
The asd gene was efficiently knocked out by the recombinant suicide vector, which replaced the asd gene with the CmR gene.
Based on the balanced lethal host-vector system, the phenotype of the Δasd mutant was further defined.
The improved method was simpler and more effective than previously reported conventional methods.
American Psychological Association (APA)
Geng, Shi-Zhong& Jiao, Xin-an& Pan, Zhi-ming& Chen, Xiao-Juan& Zhang, Xiao-Ming& Chen, Xiang. 2009. An Improved Method to Knock Out the asd Gene of Salmonella enterica Serovar Pullorum. Journal of Biomedicine and Biotechnology،Vol. 2009, no. 2009, pp.1-8.
https://search.emarefa.net/detail/BIM-487858
Modern Language Association (MLA)
Geng, Shi-Zhong…[et al.]. An Improved Method to Knock Out the asd Gene of Salmonella enterica Serovar Pullorum. Journal of Biomedicine and Biotechnology No. 2009 (2009), pp.1-8.
https://search.emarefa.net/detail/BIM-487858
American Medical Association (AMA)
Geng, Shi-Zhong& Jiao, Xin-an& Pan, Zhi-ming& Chen, Xiao-Juan& Zhang, Xiao-Ming& Chen, Xiang. An Improved Method to Knock Out the asd Gene of Salmonella enterica Serovar Pullorum. Journal of Biomedicine and Biotechnology. 2009. Vol. 2009, no. 2009, pp.1-8.
https://search.emarefa.net/detail/BIM-487858
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-487858