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Slice Cultures as a Model to Study Neurovascular Coupling and Blood Brain Barrier In Vitro
Joint Authors
Heinemann, Uwe
Papageorgiou, Ismini
Kovács, Richard
Source
Cardiovascular Psychiatry and Neurology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-02-16
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Proper neuronal functioning depends on a strictly regulated interstitial environment and tight coupling of neuronal and metabolic activity involving adequate vascular responses.
These functions take place at the blood brain barrier (BBB) composed of endothelial cells, basal lamina covered with pericytes, and the endfeet of perivascular astrocytes.
In conventional in vitro models of the BBB, some of these components are missing.
Here we describe a new model system for studying BBB and neurovascular coupling by using confocal microscopy and fluorescence staining protocols in organotypic hippocampal slice cultures.
An elaborated network of vessels is retained in culture in spite of the absence of blood flow.
Application of calcein-AM either from the interstitial or from the luminal side resulted in different staining patterns indicating the maintenance of a barrier.
By contrast, the ethidium derivative MitoSox penetrated perivascular basal lamina and revealed free radical formation in contractile cells embracing the vessels, likely pericytes.
American Psychological Association (APA)
Kovács, Richard& Papageorgiou, Ismini& Heinemann, Uwe. 2011. Slice Cultures as a Model to Study Neurovascular Coupling and Blood Brain Barrier In Vitro. Cardiovascular Psychiatry and Neurology،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-487896
Modern Language Association (MLA)
Kovács, Richard…[et al.]. Slice Cultures as a Model to Study Neurovascular Coupling and Blood Brain Barrier In Vitro. Cardiovascular Psychiatry and Neurology No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-487896
American Medical Association (AMA)
Kovács, Richard& Papageorgiou, Ismini& Heinemann, Uwe. Slice Cultures as a Model to Study Neurovascular Coupling and Blood Brain Barrier In Vitro. Cardiovascular Psychiatry and Neurology. 2011. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-487896
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-487896