Azadirachta indica Attenuates Cisplatin-Induced Nephrotoxicity and Oxidative Stress
Joint Authors
Aref, Ahmed M.
Abdel Moneim, Ahmed E.
Othman, Mohamed S.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-03
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
We investigated the effects of methanolic leaves extract of Azadirachta indica (MLEN, 500 mg/kg bwt) on cisplatin- (CP-) induced nephrotoxicity and oxidative stress in rats.
CP (5 mg/kg bwt) was injected intraperitoneally and MLEN was given by gastric gavage for 5 days before or after CP injection.
After 5 days of CP injection, CP-induced injury of the renal tissue was evidenced (i) as histopathological damage of the renal tissue, (ii) as increases in serum uric acid, urea, and creatinine, (iii) as increases in malondialdehyde (MDA) and nitric oxide (NO), (iv) as decreases in the level of glutathione and activities of superoxide dismutase, catalase, glutathione reductase, glutathione-S-transferase, and glutathione peroxidase, and (v) as increase in the expression of nuclear factor kappa B and apoptosis in kidney tissues.
However, the oral administration of MLEN to CP-intoxicated rats for 5 days brought back MDA, NO production, and enzymatic and nonenzymatic antioxidants to near normalcy.
Moreover, the histological observations evidenced that neem extract effectively rescues the kidney from CP-mediated oxidative damage.
Furthermore, PCR results for caspase-3 and caspase-9 and Bax genes showed downregulation in MLEN treated groups.
Therefore, Azadirachta indica can be considered a potential candidate for protection of nephrotoxicity induced by cisplatin.
American Psychological Association (APA)
Abdel Moneim, Ahmed E.& Othman, Mohamed S.& Aref, Ahmed M.. 2014. Azadirachta indica Attenuates Cisplatin-Induced Nephrotoxicity and Oxidative Stress. BioMed Research International،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-487907
Modern Language Association (MLA)
Abdel Moneim, Ahmed E.…[et al.]. Azadirachta indica Attenuates Cisplatin-Induced Nephrotoxicity and Oxidative Stress. BioMed Research International No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-487907
American Medical Association (AMA)
Abdel Moneim, Ahmed E.& Othman, Mohamed S.& Aref, Ahmed M.. Azadirachta indica Attenuates Cisplatin-Induced Nephrotoxicity and Oxidative Stress. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-487907
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-487907