Targeting Oxidative Stress Injury after Ischemic Stroke in Conscious Rats : Limited Benefits with Apocynin Highlight the Need to Incorporate Long Term Recovery
Joint Authors
Dusting, Gregory J.
Lin, Bin
Weston, Robert M.
Roulston, Carli L.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-01-14
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
NADPH oxidase is a major source of superoxide anion following stroke and reperfusion.
This study evaluated the effects of apocynin, a known antioxidant and inhibitor of Nox2 NADPH, on neuronal injury and cell-specific responses to stroke induced in the conscious rat.
Apocynin treatment (50 mg/kg i.p.) commencing 1 hour prior to stroke and 24 and 48 hours after stroke significantly reduced infarct volume in the cortex by ~ 60%, but had no effect on striatal damage or neurological deficits.
In situ detection of reactive oxygen species (ROS) using dihydroethidium fluorescence revealed that increased ROS detected in OX-42 positive cells following ischemia was reduced in apocynin-treated rats by ~ 51%, but surprisingly increased in surrounding NeuN positive cells of the same rats by ~ 27%, in comparison to the contralateral hemisphere.
Reduced ROS from activated microglia/macrophages treated with apocynin was associated with reduced Nox2 immunoreactivity without change to the number of cells.
These findings confirm the protective effects of apocynin and indicate a novel mechanism via reduced Nox2 expression.
We also reveal compensatory changes in neuronal ROS generation as a result of Nox2 inhibition and highlight the need to assess long term individual cell responses to inhibitors of oxidative stress.
American Psychological Association (APA)
Weston, Robert M.& Lin, Bin& Dusting, Gregory J.& Roulston, Carli L.. 2013. Targeting Oxidative Stress Injury after Ischemic Stroke in Conscious Rats : Limited Benefits with Apocynin Highlight the Need to Incorporate Long Term Recovery. Stroke Research and Treatment،Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-487992
Modern Language Association (MLA)
Weston, Robert M.…[et al.]. Targeting Oxidative Stress Injury after Ischemic Stroke in Conscious Rats : Limited Benefits with Apocynin Highlight the Need to Incorporate Long Term Recovery. Stroke Research and Treatment No. 2013 (2013), pp.1-13.
https://search.emarefa.net/detail/BIM-487992
American Medical Association (AMA)
Weston, Robert M.& Lin, Bin& Dusting, Gregory J.& Roulston, Carli L.. Targeting Oxidative Stress Injury after Ischemic Stroke in Conscious Rats : Limited Benefits with Apocynin Highlight the Need to Incorporate Long Term Recovery. Stroke Research and Treatment. 2013. Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-487992
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-487992