Partial Purification and Characterization of Rhodanese from Rainbow Trout (Oncorhynchus mykiss) Liver
Joint Authors
Tayefi-Nasrabadi, Hossein
Rahmani, Reza
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-02
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Information Technology and Computer Science
Abstract EN
Cyanide is one of the most toxic substances present in a wide variety of food materials that are consumed by animals.
Rhodanese, a ubiquitous enzyme, can catalyse the detoxification of cyanide by sulphuration reaction.
In this study, rhodanese was partially purified and characterized from the liver tissue homogenate of the rainbow trout.
The enzyme was active in a broad range of pH, from 5 to 12.
The optimal activity was found at a high pH (pH 10.5), and the temperature optimum was 25∘C.
The enzyme was heat labile, losing > 50% of relative activity after only 5 min of incubation at 40∘C.
The Km values for KCN and Na2S2O3 as substrates were 36.81 mM and 19.84 mM, respectively.
Studies on the enzyme with a number of cations showed that the activity of the enzyme was not affected by Sn2+, but Hg2+, Ba2+, Pb2+, and Ca2+ inhibited and Cu2+ activated the enzyme with a concentration-dependent manner.
American Psychological Association (APA)
Tayefi-Nasrabadi, Hossein& Rahmani, Reza. 2012. Partial Purification and Characterization of Rhodanese from Rainbow Trout (Oncorhynchus mykiss) Liver. The Scientific World Journal،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-487996
Modern Language Association (MLA)
Tayefi-Nasrabadi, Hossein& Rahmani, Reza. Partial Purification and Characterization of Rhodanese from Rainbow Trout (Oncorhynchus mykiss) Liver. The Scientific World Journal No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-487996
American Medical Association (AMA)
Tayefi-Nasrabadi, Hossein& Rahmani, Reza. Partial Purification and Characterization of Rhodanese from Rainbow Trout (Oncorhynchus mykiss) Liver. The Scientific World Journal. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-487996
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-487996